Zeta-tubulin is a member of a conserved tubulin module and is a component of the centriolar basal foot in multiciliated cells
Turk E, Wills AA, Kwon T, Sedzinski J, Wallingford JB, Stearns T (2015)
Publication Type: Journal article
Publication year: 2015
Journal
Book Volume: 25
Pages Range: 2177-2183
Journal Issue: 16
DOI: 10.1016/j.cub.2015.06.063
Abstract
There are six members of the tubulin superfamily in eukaryotes [1]. Alpha- and beta-tubulin form a heterodimer that polymerizes to form microtubules, and gamma-tubulin nucleates microtubules as a component of the gamma-tubulin ring complex. Alpha-, beta-, and gamma-tubulin are conserved in all eukaryotes. In contrast, delta- and epsilon-tubulin are conserved in many, but not all, eukaryotes and are associated with centrioles, although their molecular function is unclear [2-7]. Zeta-tubulin is the sixth and final member of the tubulin superfamily and is largely uncharacterized. We find that zeta-, epsilon-, and delta-tubulin form an evolutionarily co-conserved module, the ZED module, that has been lost at several junctions in eukaryotic evolution and that zeta- and delta-tubulin are evolutionarily interchangeable. Humans lack zeta-tubulin but have delta-tubulin. In Xenopus multiciliated cells, zeta-tubulin is a component of the basal foot, a centriolar appendage that connects centrioles to the apical cytoskeleton, and co-localizes there with epsilon-tubulin. Depletion of zeta-tubulin results in disorganization of centriole distribution and polarity in multiciliated cells. In contrast with multiciliated cells, zeta-tubulin in cycling cells does not localize to centrioles and is associated with the TRiC/CCT cytoplasmic chaperone complex. We conclude that zeta-tubulin facilitates interactions between the centrioles and the apical cytoskeleton as a component of the basal foot in differentiated cells and propose that the ZED tubulins are important for centriole functionalization and orientation of centrioles with respect to cellular polarity axes.
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United States (USA) (US)How to cite
APA:
Turk, E., Wills, A.A., Kwon, T., Sedzinski, J., Wallingford, J.B., & Stearns, T. (2015). Zeta-tubulin is a member of a conserved tubulin module and is a component of the centriolar basal foot in multiciliated cells. Current Biology, 25(16), 2177-2183. https://doi.org/10.1016/j.cub.2015.06.063
MLA:
Turk, Erin, et al. "Zeta-tubulin is a member of a conserved tubulin module and is a component of the centriolar basal foot in multiciliated cells." Current Biology 25.16 (2015): 2177-2183.
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