Validation of Dynamic Deuterium Metabolic Imaging (DMI) for the Measurement of Cerebral Metabolic Rates of Glucose in Rat

Mathy C, Thomas M, Mason G, de Graaf R, De Feyter H (2026)

Publication Type: Journal article

Publication year: 2026

Journal

NMR in Biomedicine Wiley

Book Volume: 39

Article Number: e70194

Journal Issue: 1

DOI: 10.1002/nbm.70194

Abstract

Deuterium metabolic imaging (DMI) is an innovative technique in which 2H magnetic resonance spectroscopic imaging (MRSI) is utilized to determine the metabolic activity of administered 2H-labeled substrates. As such it can be viewed as the 2H counterpart to more traditional 13C labeling methods that can be considered the gold standard for metabolic mapping in vivo. To ensure reliable findings from dynamic 2H MRSI experiments about absolute metabolic flux rates after administration of a 2H-labeled substrate it is essential to take into account 2H-specific aspects, namely 2H label losses and kinetic isotopy effects (KIEs). Here, a modified version of a 13C-based metabolic model for glucose metabolism in rat brain was developed to address these 2H-related effects, tested for 2H MRSI data acquired during infusion of [6,6'-2H2]-glucose, and validated by comparison with indirect 1H-[13C] MRSI data acquired during infusion of [1-13C]-glucose. The flux rates for glucose consumption (CMRgl = 0.57 ± 0.08 μmol/min/g) and the TCA cycle (Vtca = 1.24 ± 0.14 μmol/min/g) derived from the 2H MRSI data and using the updated metabolic model were in excellent agreement with the estimates based on 13C data (CMRgl = 0.59 ± 0.14 μmol/min/g and Vtca = 1.24 ± 0.32 μmol/min/g). The successful validation of dynamic 2H MRSI for absolute flux rate determination forms the basis for future quantitative study of metabolic disorders in vivo.

Involved external institutions

Yale University US United States (USA) (US)

How to cite

APA:

Mathy, C., Thomas, M., Mason, G., de Graaf, R., & De Feyter, H. (2026). Validation of Dynamic Deuterium Metabolic Imaging (DMI) for the Measurement of Cerebral Metabolic Rates of Glucose in Rat. NMR in Biomedicine, 39(1). https://doi.org/10.1002/nbm.70194

MLA:

Mathy, Claudius S, et al. "Validation of Dynamic Deuterium Metabolic Imaging (DMI) for the Measurement of Cerebral Metabolic Rates of Glucose in Rat." NMR in Biomedicine 39.1 (2026).

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