Effect of vacancies on blue-colored calcium phosphate scaffolds derived from Nile tilapia bone powder

Khamkongkaeo A, Wongrakpanich A, Chanamuangkon T, Chayanun S, Rojviriya C, Pimsawat A, Vongpramate D, Bootchanont A, Sailuam W, Boccaccini AR, Lohwongwatana B (2025)

Publication Type: Journal article

Publication year: 2025

Journal

Scientific Reports Nature Publishing Group: Open Access Journals - Option B

Book Volume: 15

Article Number: 24058

Journal Issue: 1

DOI: 10.1038/s41598-025-99708-z

Abstract

This study explores the fabrication and characterization of porous ceramic scaffolds using the polyurethane sponge replication technique with commercial hydroxyapatite (SA-P) and Nile Tilapia bone-derived (FB-P) powders. Scaffolds sintered at 1300 °C and 1400 °C for 5 h exhibited high porosity (70–90%) with interconnected pores. SA scaffolds exhibited greater shrinkage due to differences in particle size and morphology. FTIR and XRD analyses confirmed hydroxyapatite (HAp), β-tricalcium phosphate (β-TCP), and α-tricalcium phosphate (α-TCP) phases, with compositions influenced by sintering temperature. FB scaffolds developed a distinct blue coloration attributed to hydroxyl (OH−) and oxygen (Ov–PO4) vacancies within the HAp, while SA scaffolds appeared lighter. UV–vis and XANES analyses validated these compositional differences. In vitro cytotoxicity assays confirmed the biocompatibility of all scaffolds, with SA scaffolds exhibiting higher cell viability and proliferation than FB scaffolds, likely due to their optimized microstructure and phase composition. While FB scaffolds showed slightly lower cell proliferation, their bioactivity remained sufficient for bone tissue engineering applications. These findings suggest a promising strategy for selectively enhancing the OH− vacancy within the HAp structure and refining the HAp/β-TCP composition, thereby improving the biological performance of calcium phosphate scaffolds for biomedical applications.

Authors with CRIS profile

Aldo Boccaccini Lehrstuhl für Werkstoffwissenschaften (Biomaterialien)

Involved external institutions

Chulalongkorn University / จุฬาลงกรณ์มหาวิทยาลัย TH Thailand (TH) Mahidol University / มหาวิทยาลัยมหิดล TH Thailand (TH) Synchrotron Light Research Institute / สถาบันวิจัยแสงซินโครตรอน TH Thailand (TH) Buriram Rajabhat University / มหาวิทยาลัยราชภัฏบุรีรัมย์ TH Thailand (TH) Rajamangala University of Technology Thanyaburi (RMUTT) / มหาวิทยาลัยเทคโนโลยีราชมงคล TH Thailand (TH) Rajamangala University of Technology Isan (RMUTI) / มหาวิทยาลัยเทคโนโลยีราชมงคลอีสาน TH Thailand (TH)

How to cite

APA:

Khamkongkaeo, A., Wongrakpanich, A., Chanamuangkon, T., Chayanun, S., Rojviriya, C., Pimsawat, A.,... Lohwongwatana, B. (2025). Effect of vacancies on blue-colored calcium phosphate scaffolds derived from Nile tilapia bone powder. Scientific Reports, 15(1). https://doi.org/10.1038/s41598-025-99708-z

MLA:

Khamkongkaeo, Atchara, et al. "Effect of vacancies on blue-colored calcium phosphate scaffolds derived from Nile tilapia bone powder." Scientific Reports 15.1 (2025).

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