Arkudas A, Tjiawi J, Saumweber A, Beier J, Polykandriotis E, Bleiziffer O, Horch RE, Kneser U (2009)
Publication Type: Journal article
Publication year: 2009
Book Volume: 13
Pages Range: 2864-2874
Journal Issue: 9 A
DOI: 10.1111/j.1582-4934.2008.00410.x
Our aim was to quantitatively assess the angiogenetic effects of VEGF and bFGF immobilized in a fibrin-based drug delivery system in a suitable subcutaneous rat model. After evaluation of a suitable implantation technique (6 rats), four teflon isolation chambers containing fibrin gel matrices were implanted subcutaneously in an upside-down fashion on the back of 30 Lewis rats. The matrices consisted of 500 μl fibrin gel with two different fibrinogen concentrations (10 mg/ml or 40 mg/ml fibrinogen) and 2 I.U./ml thrombin and contained VEGF and bFGF in five different concentrations (0 to 250 ng/ml each). At 3, 7 and 14 days after implantation, matrices were explanted and subjected to histological and morphometrical analysis. At 1 week, the volume of the fibrin clots was significantly smaller in the 100 and 250 ng/ml VEGF and bFGF groups in comparison to lower concentrated growth factors. At 1 and 2 weeks, the use of growth factors in low concentrations (25 ng/ml VEGF and bFGF) significantly increased the amount of fibrovascular tissue, average fraction of blood vessels and number of blood vessels at the matrix-host interface in comparison to growth factor-free controls. Higher concentrations were neither associated with further increase of tissue formation nor with increased sprouting of blood vessels in this model. This study demonstrates that fibrin gel-immobilized angioinductive growth factors efficiently stimulate generation of fibrovascular tissue and sprouting of blood vessels in a newly developed subcutaneous upside-down isolation chamber model with an optimum between 25 and 100 ng/ml. © 2009 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd.
APA:
Arkudas, A., Tjiawi, J., Saumweber, A., Beier, J., Polykandriotis, E., Bleiziffer, O.,... Kneser, U. (2009). Evaluation of blood vessel ingrowth in fibrin gel subject to type and concentration of growth factors. Journal of Cellular and Molecular Medicine, 13(9 A), 2864-2874. https://doi.org/10.1111/j.1582-4934.2008.00410.x
MLA:
Arkudas, Andreas, et al. "Evaluation of blood vessel ingrowth in fibrin gel subject to type and concentration of growth factors." Journal of Cellular and Molecular Medicine 13.9 A (2009): 2864-2874.
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