Automatic quantitative micro-computed tomography evaluation of angiogenesis in an axially vascularized tissue-engineered bone construct
Arkudas A, Beier J, Pryymachuk G, Höreth T, Bleiziffer O, Polykandriotis E, Heß A, Gulle H, Horch RE, Kneser U (2010)
Publication Type: Journal article
Publication year: 2010
Journal
Book Volume: 16
Pages Range: 1503-1514
Journal Issue: 6
DOI: 10.1089/ten.tec.2010.0016
Abstract
Introduction: We invented an automatic observer-independent quantitative method to analyze vascularization using micro-computed tomography (CT) along with three-dimensional (3D) reconstruction in a tissue engineering model. Materials and Methods: An arteriovenous loop was created in the medial thigh of 30 rats and was placed in a particulated porous hydroxyapatite and beta-tricalcium phosphate matrix, filled with fibrin (10mg/mL fibrinogen and 2IU/mL thrombin) without (group A) or with (group B) application of fibrin-gel-immobilized angiogenetic growth factors vascular endothelial growth factor (VEGF165) and basic fibroblast growth factor (bFGF). The explantation intervals were 2, 4, and 8 weeks. Specimens were investigated by means of micro-CT followed by an automatic 3D analysis, which was correlated to histomorphometrical findings. Results: In both groups, the arteriovenous loop led to generation of dense vascularized connective tissue with differentiated and functional vessels inside the matrix. Quantitative analysis of vascularization using micro-CT showed to be superior to histological analysis. The micro-CT analysis also allows the assessment of different other, more complex vascularization parameters within 3D constructs, demonstrating an early improvement of vascularization by application of fibrin-gel-immobilized VEGF165 and bFGF. Conclusions: In this study quantitative analysis of vascularization using micro-CT along with 3D reconstruction and automatic analysis exhibit to be a powerful method superior to histological evaluation of cross sections. © Copyright 2010, Mary Ann Liebert, Inc.
Authors with CRIS profile
Andreas Arkudas
Medizinische Fakultät
Tobias Höreth
Medizinische Fakultät
Elias Polykandriotis
Medizinische Fakultät
Andreas Heß
Lehrstuhl für Pharmakologie und Toxikologie
Raymund Horch
Professur für Plastische Chirurgie und Handchirurgie
Involved external institutions
Austria (AT)How to cite
APA:
Arkudas, A., Beier, J., Pryymachuk, G., Höreth, T., Bleiziffer, O., Polykandriotis, E.,... Kneser, U. (2010). Automatic quantitative micro-computed tomography evaluation of angiogenesis in an axially vascularized tissue-engineered bone construct. Tissue Engineering - Part C: Methods, 16(6), 1503-1514. https://doi.org/10.1089/ten.tec.2010.0016
MLA:
Arkudas, Andreas, et al. "Automatic quantitative micro-computed tomography evaluation of angiogenesis in an axially vascularized tissue-engineered bone construct." Tissue Engineering - Part C: Methods 16.6 (2010): 1503-1514.
BibTeX: Download