Hyaluronan-based heparin-incorporated hydrogels for generation of axially vascularized bioartificial bone tissues: In vitro and in vivo evaluation in a PLDLLA-TCP-PCL-composite system
Rath SN, Pryymachuk G, Bleiziffer O, Lam CX, Arkudas A, Ho ST, Beier J, Horch RE, Hutmacher DW, Kneser U (2011)
Publication Language: English
Publication Type: Journal article
Publication year: 2011
Journal
Book Volume: 22
Pages Range: 1279-1291
Journal Issue: 5
DOI: 10.1007/s10856-011-4300-0
Abstract
Smart matrices are required in bone tissueengineered grafts that provide an optimal environment for cells and retain osteo-inductive factors for sustained biological activity. We hypothesized that a slow-degrading heparin-incorporated hyaluronan (HA) hydrogel can preserve BMP-2; while an arterio-venous (A-V) loop can support axial vascularization to provide nutrition for a bioartificial bone graft. HA was evaluated for osteoblast growth and BMP-2 release. Porous PLDLLA-TCP-PCL scaffolds were produced by rapid prototyping technology and applied in vivo along with HA-hydrogel, loaded with either primary osteoblasts or BMP-2. A microsurgically created A-V loop was placed around the scaffold, encased in an isolation chamber in Lewis rats. HA-hydrogel supported growth of osteoblasts over 8 weeks and allowed sustained release of BMP-2 over 35 days. The A-V loop provided an angiogenic stimulus with the formation of vascularized tissue in the scaffolds. Bone-specific genes were detected by real time RT-PCR after 8 weeks. However, no significant amount of bone was observed histologically. The heterotopic isolation chamber in combination with absent biomechanical stimulation might explain the insufficient bone formation despite adequate expression of bone-related genes. Optimization of the interplay of osteogenic cells and osteo-inductive factors might eventually generate sufficient amounts of axially vascularized bone grafts for reconstructive surgery. © Springer Science+Business Media, LLC 2011.
Authors with CRIS profile
Andreas Arkudas
Department of Plastic and Hand Surgery
Raymund Horch
Professur für Plastische Chirurgie und Handchirurgie
Involved external institutions
National University of Singapore (NUS)
Singapore (SG)
NUS Yong Loo Lin School of Medicine
Singapore (SG)
Queensland University of Technology (QUT)
Australia (AU)
Singapore (SG)
NUS Yong Loo Lin School of Medicine
Singapore (SG)
Queensland University of Technology (QUT)
Australia (AU)
How to cite
APA:
Rath, S.N., Pryymachuk, G., Bleiziffer, O., Lam, C.X., Arkudas, A., Ho, S.T.,... Kneser, U. (2011). Hyaluronan-based heparin-incorporated hydrogels for generation of axially vascularized bioartificial bone tissues: In vitro and in vivo evaluation in a PLDLLA-TCP-PCL-composite system. Journal of Materials Science: Materials in Medicine, 22(5), 1279-1291. https://doi.org/10.1007/s10856-011-4300-0
MLA:
Rath, Subha N., et al. "Hyaluronan-based heparin-incorporated hydrogels for generation of axially vascularized bioartificial bone tissues: In vitro and in vivo evaluation in a PLDLLA-TCP-PCL-composite system." Journal of Materials Science: Materials in Medicine 22.5 (2011): 1279-1291.
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