Lectin histochemistry shows WGA, PHA-L and HPA binding increases during progression of human colorectal cancer
Hägerbäumer P, Vieth M, Anders M, Schumacher U (2015)
Publication Type: Journal article
Publication year: 2015
Journal
Book Volume: 35
Pages Range: 5333-5340
Journal Issue: 10
Abstract
Background/Aim: Most colorectal carcinomas develop from an adenoma-carcinoma sequence to metastatic disease. The aim of the present study was to use lectins, carbohydrate-binding proteins, to detect changes in glycosylation during this malignant progression. Materials and Methods: Sections from normal colorectal mucosa, highgrade intraepithelial neoplasia, submucosal colorectal carcinoma and metastases from patients who underwent colorectal surgery were stained by lectins with different sugar specificities namely agglutins from Wheatgerm (WGA), Helix pomatia (HPA), Phaseolus vulgaris (PHA-L), Ulex europaeus (UEA-I), Sambucus nigra (SNA-I), Canavalia ensiformis (Con A), Galanthus nivalis (GNA) and Dolichos biflorus (DBA). Results: Binding patterns of all lectins except SNA-I, Con A and DBA changed during the adenoma-carcinoma sequence. Conclusion: The results indicate that lectins specific for mannose, N-acetylgalactosamine, N-acetylglucosamine, sialic acid, β-1,6-branched oligosaccharides and α-1-fucose may be associated with malignant progression.
Involved external institutions
Germany (DE)How to cite
APA:
Hägerbäumer, P., Vieth, M., Anders, M., & Schumacher, U. (2015). Lectin histochemistry shows WGA, PHA-L and HPA binding increases during progression of human colorectal cancer. Anticancer Research, 35(10), 5333-5340.
MLA:
Hägerbäumer, Pia, et al. "Lectin histochemistry shows WGA, PHA-L and HPA binding increases during progression of human colorectal cancer." Anticancer Research 35.10 (2015): 5333-5340.
BibTeX: Download