Expression profiles of cancer stem cell markers: CD133, CD44, Musashi-1 and EpCAM in the cardiac mucosa—Barrett's esophagus—early esophageal adenocarcinoma—advanced esophageal adenocarcinoma sequence

Mokrowiecka A, Veits L, Falkeis C, Musial J, Kordek R, Lochowski M, Kozak J, Wierzchniewska-Lawska A, Vieth M, Malecka-Panas E (2017)

Publication Type: Journal article

Publication year: 2017

Journal

Pathology Research and Practice Elsevier

Book Volume: 213

Pages Range: 205-209

Journal Issue: 3

DOI: 10.1016/j.prp.2016.12.018

Abstract

Introduction Barrett's esophagus (BE), which develops as a result of gastroesophageal reflux disease, is a preneoplastic condition for esophageal adenocarcinoma (EAC). A new hypothesis suggests that cancer is a disease of stem cells, however, their expression and pathways in BE − EAC sequence are not fully elucidated yet. Aims We used a panel of putative cancer stem cells markers to identify stem cells in consecutive steps of BE-related cancer progression. Methods Immunohistochemistry was performed on formalin-fixed, paraffin-embedded blocks from 58 patients with normal cardiac mucosa (n = 5), BE (n = 14), early EAC (pT1) from mucosal resection (n = 17) and advanced EAC (pT1-T4) from postoperative specimens (n = 22). Expression of the CD133, CD44, Musashi-1 and EpCAM was analyzed using respective monoclonal antibodies. Results All markers showed a heterogeneous expression pattern, mainly at the base of the crypts of Barrett's epithelium and EAC, with positive stromal cells in metaplastic and dysplastic lesions. Immuno-expression of EpCAM, CD44 and CD133 in cardiac mucosa was significantly lower (mean immunoreactivity score (IRS) = 1.2; 0.0; 0.4; respectively) compared to their expression in Barrett's metaplasia (mean IRS = 4.3; 0.14; 0.7; respectively), in early adenocarcinoma (mean IRS = 4.4; 0.29; 1.3; respectively) and in advanced adenocarcinoma (mean IRS = 6.6; 0.7; 2.7; respectively) (p <0.05). On the contrary, Musashi-1 expression was higher in BE and early ADC compared to GM and advanced ADC (NS). Conclusion Our results suggest that the stem cells could be present in premalignant lesions. EpCAM, CD44 and CD133 expression could be candidate markers for BE progression, whereas Musashi-1 may be a marker of the small intestinal features of Barrett's mucosa.

Involved external institutions

Medical University of Łódź / Uniwersytet Medyczny w Łodzi PL Poland (PL) Klinikum Bayreuth DE Germany (DE)

How to cite

APA:

Mokrowiecka, A., Veits, L., Falkeis, C., Musial, J., Kordek, R., Lochowski, M.,... Malecka-Panas, E. (2017). Expression profiles of cancer stem cell markers: CD133, CD44, Musashi-1 and EpCAM in the cardiac mucosa—Barrett's esophagus—early esophageal adenocarcinoma—advanced esophageal adenocarcinoma sequence. Pathology Research and Practice, 213(3), 205-209. https://doi.org/10.1016/j.prp.2016.12.018

MLA:

Mokrowiecka, Anna, et al. "Expression profiles of cancer stem cell markers: CD133, CD44, Musashi-1 and EpCAM in the cardiac mucosa—Barrett's esophagus—early esophageal adenocarcinoma—advanced esophageal adenocarcinoma sequence." Pathology Research and Practice 213.3 (2017): 205-209.

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