The effects of a plant proteinase inhibitor from Enterolobium contortisiliquum on human tumor cell lines

Nakahata AM, Mayer B, Ries C, De Paula CAA, Karow M, Neth P, Sampaio MU, Jochum M, Oliva MLV (2011)

Publication Type: Journal article

Publication year: 2011

Journal

Biological Chemistry Walter de Gruyter

Book Volume: 392

Pages Range: 327-336

Journal Issue: 4

DOI: 10.1515/bc.2011.031

Abstract

Supplementary to the efficient inhibition of trypsin, chymotrypsin, plasma kallikrein, and plasmin already described by the EcTI inhibitor from Enterolobium contortisiliquum, it also blocks human neutrophil elastase (K iapps4.3 nM) and prevents phorbol ester (PMA)-stimulated activation of matrix metalloproteinase (MMP)-2 probably via interference with membrane-type 1 (MT1)-MMP. Moreover, plasminogeninduced activation of proMMP-9 and processing of active MMP-2 was also inhibited. Furthermore, the effect of EcTI on the human cancer cell lines HCT116 and HT29 (colorectal), SkBr-3 and MCF-7 (breast), K562 and THP-1 (leukemia), as well as on human primary fibroblasts and human mesenchymal stem cells (hMSCs) was studied. EcTI inhibited in a concentration range of 1.0-2.5 μM rather specifically tumor cell viability without targeting primary fibroblasts and hMSCs. Taken together, our data indicate that the polyspecific proteinase inhibitor EcTI prevents proMMPactivation and is cytotoxic against tumor cells without affecting normal tissue remodeling fibroblasts or regenerative hMSCs being an important tool in the studies of tumor cell development and dissemination. Copyright © by Walter de Gruyter.

Involved external institutions

Federal University of São Paulo / Universidade Federal de São Paulo (UNIFESP) BR Brazil (BR) Ludwig-Maximilians-Universität (LMU) DE Germany (DE) Klinikum der Universität München DE Germany (DE)

How to cite

APA:

Nakahata, A.M., Mayer, B., Ries, C., De Paula, C.A.A., Karow, M., Neth, P.,... Oliva, M.L.V. (2011). The effects of a plant proteinase inhibitor from Enterolobium contortisiliquum on human tumor cell lines. Biological Chemistry, 392(4), 327-336. https://doi.org/10.1515/bc.2011.031

MLA:

Nakahata, Adriana Miti, et al. "The effects of a plant proteinase inhibitor from Enterolobium contortisiliquum on human tumor cell lines." Biological Chemistry 392.4 (2011): 327-336.

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