Identification of the Heptapeptide PR7 and Octapeptide SF8 With Potent Antioxidative and Angiotensin Converting Enzyme Inhibitory Activity From the Fermented Dairy By-Product “Buttermilk”

Magouz O, Ibrahim MA, Mehanna N, Gensberger-Reigl S, Dalabasmaz S, Pischetsrieder M (2024)

Publication Type: Journal article

Publication year: 2024

Journal

Food Frontiers Wiley

DOI: 10.1002/fft2.538

Abstract

The present study aimed to identify antioxidative and angiotensin converting enzyme (ACE) inhibitory peptides in fermented buttermilk after simulated gastric digestion. Following isoelectric focusing fractionation, peptides of the most active fractions were identified by ultrahigh performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS). Subsequently, 23 peptides were synthesized and tested for antioxidative and ACE inhibitory activity. The peptide PR7 (PWDQVKR, αs2-casein 108–114) exerted comparable antioxidative activity to ascorbic acid, whereas the peptide SF8 (SGPLRPFF, butyrophilin 427–434) efficiently inhibited ACE with an IC50 value of 1.57 µM. The binding modes of the five most active ACE inhibitors were predicted using AlphaFold2 multimer software. Molecular mechanics (MM) minimization and molecular dynamics (MD) simulations of all complexes, followed by MM-generalized born surface area (GBSA) binding energy calculations, were predicted. Notably, these peptides manifested good binding affinities to ACE. The estimated MM-GBSA/MD binding energies correlated with the detected IC50 values [correlation coefficient (R2) = 0.79]. Post-MD analyses over 100 ns elucidated the steadiness of the five peptides.

Authors with CRIS profile

Sabrina Gensberger-Reigl Lehrstuhl für Lebensmittelchemie (Henriette-Schmidt-Burkhardt Lehrstuhl) Sevim Dalabasmaz Lehrstuhl für Lebensmittelchemie (Henriette-Schmidt-Burkhardt Lehrstuhl) Monika Pischetsrieder Lehrstuhl für Lebensmittelchemie (Henriette-Schmidt-Burkhardt Lehrstuhl)

Additional Organisation(s)

FAU Forschungszentrum Neue Wirkstoffe (FAU NeW)

Involved external institutions

Pennsylvania State University (Penn State) US United States (USA) (US) Kafr el-Sheikh University EG Egypt (EG)

How to cite

APA:

Magouz, O., Ibrahim, M.A., Mehanna, N., Gensberger-Reigl, S., Dalabasmaz, S., & Pischetsrieder, M. (2024). Identification of the Heptapeptide PR7 and Octapeptide SF8 With Potent Antioxidative and Angiotensin Converting Enzyme Inhibitory Activity From the Fermented Dairy By-Product “Buttermilk”. Food Frontiers. https://doi.org/10.1002/fft2.538

MLA:

Magouz, Osama, et al. "Identification of the Heptapeptide PR7 and Octapeptide SF8 With Potent Antioxidative and Angiotensin Converting Enzyme Inhibitory Activity From the Fermented Dairy By-Product “Buttermilk”." Food Frontiers (2024).

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