A Network Biology-Guided Investigation of the Long Noncoding RNAs’ Role in Glaucoma

Choudhari JK, Vera J, Chatterjee T (2025)

Publication Type: Book chapter / Article in edited volumes

Publication year: 2025

Journal

Methods in Molecular Biology Humana Press

Publisher: Humana Press Inc.

Series: Methods in Molecular Biology

Book Volume: 2883

Pages Range: 427-453

DOI: 10.1007/978-1-0716-4290-0_19

Abstract

Glaucoma is a group of eye diseases characterized by progressive harm to the optic nerve that often results in loss of vision and blindness. Recent studies suggest that deregulation in the expression of a particular type of RNAs named long noncoding RNAs (lncRNAs) can impact the development of glaucoma and, hence, are promising targets for drug discovery. LncRNAs are transcripts with a length longer than 200 nucleotides that are not translated into proteins. Their extended length differentiates them from other non-coding RNAs such as microRNAs. Furthermore, lncRNAs exhibit diverse and often unpredictable regulatory functions within gene networks, unlike the well-defined gene silencing role of microRNAs. This chapter aims to discuss the role of lncRNAs in the pathogenesis of glaucoma. We will discuss and investigate lncRNAs linked to glaucoma, examine their functional regulation mechanisms in (glaucoma) gene networks, the extent of their (abnormal) expression in the disease, and their prospective as therapeutic targets. To this end, we performed lncRNA expression analysis from publicly available datasets and utilized this information to investigate their role in key pathways and biological mechanisms contributing to glaucoma progression. We employed a computational pipeline to construct glaucoma-associated lncRNA–miRNA, miRNA–TFs, gene–miRNA, protein–TF, and TF–TFs gene networks and used them to identify lncRNAs biomarkers for early diagnosis and disease progression monitoring. We identified lncRNA-enriched regulatory clusters and explored the potential of some of these lncRNA–gene clusters as targets for new therapies for glaucoma. Using this approach, we expect to give initial steps towards the elucidation of lncRNAs’ role in glaucoma onset and progression.

Authors with CRIS profile

Julio Vera González Professur für Tumorimmunologie (Schwerpunkt Systembiologie)

Involved external institutions

Maulana Azad National Institute of Technology (MANIT) IN India (IN) Raipur Institute of Technology IN India (IN)

How to cite

APA:

Choudhari, J.K., Vera, J., & Chatterjee, T. (2025). A Network Biology-Guided Investigation of the Long Noncoding RNAs’ Role in Glaucoma. In (pp. 427-453). Humana Press Inc..

MLA:

Choudhari, Jyoti Kant, Julio Vera, and Tanushree Chatterjee. "A Network Biology-Guided Investigation of the Long Noncoding RNAs’ Role in Glaucoma." Humana Press Inc., 2025. 427-453.

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