Herpes Simplex Virus Type 1 Propagation, Titration and Single-step Growth Curves
Grosche L, Döhner K, Düthorn A, Hickford-Martinez A, Steinkasserer A, Sodeik B (2019)
Publication Type: Journal article
Publication year: 2019
Journal
Book Volume: 9
Article Number: e3441
Journal Issue: 23
Abstract
Given the endemic seroprevalence of herpes simplex viruses (HSV), its associated human diseases, and the emergence of acyclovir-resistant strains, there is a continuous need for better antiviral therapies. Towards this aim, identifying mechanistic details of how HSV-1 manipulates infected cells, how it modulates the immune responses, and how it causes diseases are essential. Measuring titers and growth kinetics of clinical isolates and viral mutants are important for a thorough characterization of viral phenotypes in vitro and in vivo. We provide protocols for the preparation as well as titration of HSV-1 stocks, and explain how to perform single-step growth curves to characterize the functions of viral proteins or host factors during infection. In particular, we describe methods to prepare and characterize high-titer HSV-1 stocks with low genome to titer ratios that are required for infection studies in cell culture and animal experiments.
Authors with CRIS profile
Alexandra Birzer
Department of Immune Modulation
Alexander Steinkasserer
Professur für Experimentelle Dermatologie
Involved external institutions
Germany (DE)How to cite
APA:
Grosche, L., Döhner, K., Düthorn, A., Hickford-Martinez, A., Steinkasserer, A., & Sodeik, B. (2019). Herpes Simplex Virus Type 1 Propagation, Titration and Single-step Growth Curves. Bio-protocol, 9(23). https://doi.org/10.21769/BioProtoc.3441
MLA:
Grosche, Linda, et al. "Herpes Simplex Virus Type 1 Propagation, Titration and Single-step Growth Curves." Bio-protocol 9.23 (2019).
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