Tuning nanoparticle uptake: Live-cell imaging reveals two distinct endocytosis mechanisms mediated by natural and artificial EGFR targeting ligand
Mickler FM, Möckl L, Ruthardt N, Ogris M, Wagner E, Bräuchle C (2012)
Publication Type: Journal article
Publication year: 2012
Journal
Book Volume: 12
Pages Range: 3417-3423
Journal Issue: 7
DOI: 10.1021/nl300395q
Abstract
Therapeutic nanoparticles can be directed to cancer cells by incorporating selective targeting ligands. Here, we investigate the epidermal growth factor receptor (EGFR)-mediated endocytosis of gene carriers (polyplexes) either targeted with natural EGF or GE11, a short synthetic EGFR-binding peptide. Highly sensitive live-cell fluorescence microcopy with single particle resolution unraveled the existence of two different uptake mechanisms; EGF triggers accelerated nanoparticle endocytosis due to its dual active role in receptor binding and signaling activation. For GE11, an alternative EGFR signaling independent, actin-driven pathway is presented. © 2012 American Chemical Society.
Involved external institutions
Germany (DE)How to cite
APA:
Mickler, F.M., Möckl, L., Ruthardt, N., Ogris, M., Wagner, E., & Bräuchle, C. (2012). Tuning nanoparticle uptake: Live-cell imaging reveals two distinct endocytosis mechanisms mediated by natural and artificial EGFR targeting ligand. Nano Letters, 12(7), 3417-3423. https://doi.org/10.1021/nl300395q
MLA:
Mickler, Frauke M., et al. "Tuning nanoparticle uptake: Live-cell imaging reveals two distinct endocytosis mechanisms mediated by natural and artificial EGFR targeting ligand." Nano Letters 12.7 (2012): 3417-3423.
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