Microdomain Formation Controls Spatiotemporal Dynamics of Cell-Surface Glycoproteins
Möckl L, Horst AK, Kolbe K, Lindhorst TK, Bräuchle C (2015)
Publication Type: Journal article
Publication year: 2015
Journal
Book Volume: 16
Pages Range: 2023-2028
Journal Issue: 14
Abstract
The effect of galectin-mediated microdomain formation on the spatiotemporal dynamics of glycosylated membrane proteins in human microvascular endothelial cells (HMEC-1) was studied qualitatively and quantitatively by high-resolution fluorescence microscopy and artificially mimicked by metabolic glycoprotein engineering. Two types of membrane proteins, sialic acid-bearing proteins (SABPs) and mucin-type proteins (MTPs), were investigated. For visualization they were metabolically labeled with azido sugars and then coupled to a cyclooctyne-conjugated fluorescent dye by click chemistry. Both spatial (diffusion) and temporal (residence time) dynamics of SABPs and MTPs on the membrane were investigated after treatment with exogenous galectin-1 or -3. Strong effects of galectin-mediated lattice formation were observed for MTPs (decreased spatial mobility), but not for SABPs. Lattice formation also strongly decreased the turnover of MTPs (increased residence time on the cell membrane). The effects of galectin-mediated crosslinking was accurately mimicked by streptavidin-mediated crosslinking of biotin-tagged glycoproteins and verified by single-molecule tracking. This technique allows the induction of crosslinking of membrane proteins under precisely controlled conditions, thereby influencing membrane residence time and the spatial dynamics of glycans on the cell membrane in a controlled way.
Involved external institutions
Ludwig-Maximilians-Universität (LMU)
Germany (DE)
Universitätsklinikum Hamburg-Eppendorf (UKE)
Germany (DE)
Christian-Albrechts-Universität zu Kiel
Germany (DE)
Germany (DE)
Universitätsklinikum Hamburg-Eppendorf (UKE)
Germany (DE)
Christian-Albrechts-Universität zu Kiel
Germany (DE)
How to cite
APA:
Möckl, L., Horst, A.K., Kolbe, K., Lindhorst, T.K., & Bräuchle, C. (2015). Microdomain Formation Controls Spatiotemporal Dynamics of Cell-Surface Glycoproteins. ChemBioChem, 16(14), 2023-2028. https://doi.org/10.1002/cbic.201500361
MLA:
Möckl, Leonhard, et al. "Microdomain Formation Controls Spatiotemporal Dynamics of Cell-Surface Glycoproteins." ChemBioChem 16.14 (2015): 2023-2028.
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