Artificial Formation and Tuning of Glycoprotein Networks on Live Cell Membranes: A Single-Molecule Tracking Study
Möckl L, Lindhorst TK, Bräuchle C (2016)
Publication Type: Journal article
Publication year: 2016
Journal
Book Volume: 17
Pages Range: 829-835
Journal Issue: 6
Abstract
We present a method to artificially induce network formation of membrane glycoproteins and show the precise tuning of their interconnection on living cells. For this, membrane glycans are first metabolically labeled with azido sugars and then tagged with biotin by copper-free click chemistry. Finally, these biotin-tagged membrane proteins are interconnected with streptavidin (SA) to form an artificial protein network in analogy to a lectin-induced lattice. The degree of network formation can be controlled by the concentration of SA, its valency, and the concentration of biotin on membrane proteins. This was verified by investigation of the spatiotemporal dynamics of the SA-protein networks employing single-molecule tracking. It was also proven that this network formation strongly influences the biologically relevant process of endocytosis as it is known from natural lattices on the cell surface.
Involved external institutions
Ludwig-Maximilians-Universität (LMU)
Germany (DE)
Christian-Albrechts-Universität zu Kiel
Germany (DE)
Germany (DE)
Christian-Albrechts-Universität zu Kiel
Germany (DE)
How to cite
APA:
Möckl, L., Lindhorst, T.K., & Bräuchle, C. (2016). Artificial Formation and Tuning of Glycoprotein Networks on Live Cell Membranes: A Single-Molecule Tracking Study. ChemPhysChem, 17(6), 829-835. https://doi.org/10.1002/cphc.201500809
MLA:
Möckl, Leonhard, Thisbe K. Lindhorst, and Christoph Bräuchle. "Artificial Formation and Tuning of Glycoprotein Networks on Live Cell Membranes: A Single-Molecule Tracking Study." ChemPhysChem 17.6 (2016): 829-835.
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