En route from artificial to natural: Evaluation of inhibitors of mannose-specific adhesion of E. coli under flow

Möckl L, Fessele C, Despras G, Bräuchle C, Lindhorst TK (2016)

Publication Type: Journal article

Publication year: 2016

Journal

Biochimica et Biophysica Acta - General Subjects Elsevier

Book Volume: 1860

Pages Range: 2031-2036

Journal Issue: 9

DOI: 10.1016/j.bbagen.2016.06.021

Abstract

We investigated the properties of six Escherichia coli adhesion inhibitors under static and under flow conditions. On mannan-covered model substrates and under static conditions, all inhibitors were able to almost completely abolish lectin-mediated E. coli adhesion. On a monolayer of living human microvascular endothelial cells (HMEC-1), the inhibitors reduced adhesion under static conditions as well, but a large fraction of bacteria still managed to adhere even at highest inhibitor concentrations. In contrast, under flow conditions E. coli did not exhibit any adhesion to HMEC-1 not even at inhibitor concentrations where significant adhesion was detected under static conditions. This indicates that the presence of shear stress strongly affects inhibitor properties and must be taken into account when evaluating the potency of bacterial adhesion inhibitors.

Involved external institutions

Christian-Albrechts-Universität zu Kiel DE Germany (DE) Ludwig-Maximilians-Universität (LMU) DE Germany (DE)

How to cite

APA:

Möckl, L., Fessele, C., Despras, G., Bräuchle, C., & Lindhorst, T.K. (2016). En route from artificial to natural: Evaluation of inhibitors of mannose-specific adhesion of E. coli under flow. Biochimica et Biophysica Acta - General Subjects, 1860(9), 2031-2036. https://doi.org/10.1016/j.bbagen.2016.06.021

MLA:

Möckl, Leonhard, et al. "En route from artificial to natural: Evaluation of inhibitors of mannose-specific adhesion of E. coli under flow." Biochimica et Biophysica Acta - General Subjects 1860.9 (2016): 2031-2036.

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