Azido Pentoses: A New Tool To Efficiently Label Mycobacterium tuberculosis Clinical Isolates
Kolbe K, Möckl L, Sohst V, Brandenburg J, Engel R, Malm S, Bräuchle C, Holst O, Lindhorst TK, Reiling N (2017)
Publication Type: Journal article
Publication year: 2017
Journal
Book Volume: 18
Pages Range: 1172-1176
Journal Issue: 13
Abstract
Mycobacterium tuberculosis (Mtb), the main causative agent of tuberculosis (Tb), has a complex cell envelope which forms an efficient barrier to antibiotics, thus contributing to the challenges of anti-tuberculosis therapy. However, the unique Mtb cell wall can be considered an advantage and be utilized to selectively label Mtb bacteria. Here we introduce three azido pentoses as new compounds for metabolic labeling of Mtb: 3-azido arabinose (3AraAz), 3-azido ribose (3RiboAz), and 5-azido arabinofuranose (5AraAz). 5AraAz demonstrated the highest level of Mtb labeling and was efficiently incorporated into the Mtb cell wall. All three azido pentoses can be easily used to label a variety of Mtb clinical isolates without influencing Mtb-dependent phagosomal maturation arrest in infection studies with human macrophages. Thus, this metabolic labeling method offers the opportunity to attach desired molecules to the surface of Mtb bacteria in order to facilitate investigation of the varying virulence characteristics of different Mtb clinical isolates, which influence the outcome of a Tb infection.
Involved external institutions
Forschungszentrum Borstel - Leibniz-Zentrum für Medizin und Biowissenschaften / Research Center Borstel - Leibniz-Center for Medicine and Biosciences
Germany (DE)
Christian-Albrechts-Universität zu Kiel
Germany (DE)
Ludwig-Maximilians-Universität (LMU)
Germany (DE)
Germany (DE)
Christian-Albrechts-Universität zu Kiel
Germany (DE)
Ludwig-Maximilians-Universität (LMU)
Germany (DE)
How to cite
APA:
Kolbe, K., Möckl, L., Sohst, V., Brandenburg, J., Engel, R., Malm, S.,... Reiling, N. (2017). Azido Pentoses: A New Tool To Efficiently Label Mycobacterium tuberculosis Clinical Isolates. ChemBioChem, 18(13), 1172-1176. https://doi.org/10.1002/cbic.201600706
MLA:
Kolbe, Katharina, et al. "Azido Pentoses: A New Tool To Efficiently Label Mycobacterium tuberculosis Clinical Isolates." ChemBioChem 18.13 (2017): 1172-1176.
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