Dendrimer-Based Signal Amplification of Click-Labelled DNA in Situ
Raddaoui N, Stazzoni S, Möckl L, Viverge B, Geiger F, Engelke H, Bräuchle C, Carell T (2017)
Publication Type: Journal article
Publication year: 2017
Journal
Book Volume: 18
Pages Range: 1716-1720
Journal Issue: 17
Abstract
The in vivo incorporation of alkyne-modified bases into the genome of cells is today the basis for the efficient detection of cell proliferation. Cells are grown in the presence of ethinyl-dU (EdU), fixed and permeabilised. The incorporated alkynes are then efficiently detected by using azide-containing fluorophores and the CuI-catalysed alkyne–azide click reaction. For a world in which constant improvement in the sensitivity of a given method is driving diagnostic advancement, we developed azide- and alkyne-modified dendrimers that allow the establishment of sandwich-type detection assays that show significantly improved signal intensities and signal-to-noise ratios far beyond that which is currently possible.
Authors with CRIS profile
Involved external institutions
Germany (DE)How to cite
APA:
Raddaoui, N., Stazzoni, S., Möckl, L., Viverge, B., Geiger, F., Engelke, H.,... Carell, T. (2017). Dendrimer-Based Signal Amplification of Click-Labelled DNA in Situ. ChemBioChem, 18(17), 1716-1720. https://doi.org/10.1002/cbic.201700209
MLA:
Raddaoui, Nada, et al. "Dendrimer-Based Signal Amplification of Click-Labelled DNA in Situ." ChemBioChem 18.17 (2017): 1716-1720.
BibTeX: Download