Activation of GPR35 protects against cerebral ischemia by recruiting monocyte-derived macrophages
Sharmin O, Abir AH, Potol A, Alam M, Banik J, Rahman T, Tarannum N, Wadud R, Habib ZF, Rahman M (2020)
Publication Type: Journal article, Original article
Publication year: 2020
Journal
Book Volume: 10
Article Number: 9400
Issue: 1
DOI: 10.1038/s41598-020-66417-8
Abstract
Pamoic acid is a potent ligand for G protein Coupled Receptor 35 (GPR35) and exhibits antinociceptive property. GPR35 activation leads to increased energy utilization and the expression of anti-inflammatory genes. However, its role in brain disorders, especially in stroke, remains unexplored. Here we show in a mouse model of stroke that GPR35 activation by pamoic acid is neuroprotective. Pharmacological inhibition of GPR35 reveals that pamoic acid reduces infarcts size in a GPR35 dependent manner. The flowcytometric analysis shows the expression of GPR35 on the infiltrating monocytes/macrophages and neutrophils in the ischemic brain. Pamoic acid treatment results in a preferential increment of noninflammatory Ly-6CLo monocytes/macrophages in the ischemic brain along with the reduced neutrophil counts. The neuroprotective effect of GPR35 activation depends on protein kinase B (Akt) and p38 MAPK. Together we conclude that GPR35 activation by pamoic acid reprograms Ly-6CLo monocytes/macrophages to relay a neuroprotective signal into the ischemic brain.
Involved external institutions
Bangladesh (BD)How to cite
APA:
Sharmin, O., Abir, A.H., Potol, A., Alam, M., Banik, J., Rahman, T.,... Rahman, M. (2020). Activation of GPR35 protects against cerebral ischemia by recruiting monocyte-derived macrophages. Scientific Reports, 10. https://doi.org/10.1038/s41598-020-66417-8
MLA:
Sharmin, Ozayra, et al. "Activation of GPR35 protects against cerebral ischemia by recruiting monocyte-derived macrophages." Scientific Reports 10 (2020).
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