Genetic and cellular analyses of zebrafish atrioventricular cushion and valve development
Beis D, Bartman T, Jin SW, Scott IC, D'Amico L, Ober EA, Verkade H, Frantsve J, Field HA, Wehman AM, Baier H, Tallafuss A, Bally-Cuif L, Chen JN, Stainier DYR, Jungblut B (2005)
Publication Language: English
Publication Type: Journal article
Publication year: 2005
Journal
Book Volume: 132
Pages Range: 4193-4204
Journal Issue: 18
DOI: 10.1242/dev.01970
Abstract
Defects in cardiac valve morphogenesis and septation of the heart chambers constitute some of the most common human congenital abnormalities. Some of these defects originate from errors in atrioventricular (AV) endocardial cushion development. Although this process is being extensively studied in mouse and chick, the zebrafish system presents several advantages over these models, including the ability to carry out forward genetic screens and study vertebrate gene function at the single cell level. In this paper, we analyze the cellular and subcellular architecture of the zebrafish heart during stages of AV cushion and valve development and gain an unprecedented level of resolution into this process. We find that endocardial cells in the AV canal differentiate morphologically before the onset of epithelial to mesenchymal transformation, thereby defining a previously unappreciated step during AV valve formation. We use a combination of novel transgenic lines and fluorescent immunohistochemistry to analyze further the role of various genetic (Notch and Calcineurin signaling) and epigenetic (heart function) pathways in this process. In addition, from a large-scale forward genetic screen we identified 55 mutants, defining 48 different genes, that exhibit defects in discrete stages of AV cushion development. This collection of mutants provides a unique set of tools to further our understanding of the genetic basis of cell behavior and differentiation during AV valve development.
Authors with CRIS profile
Involved external institutions
University of California San Francisco (UCSF)
United States (USA) (US)
Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich
Germany (DE)
University of California Los Angeles (UCLA)
United States (USA) (US)
United States (USA) (US)
Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich
Germany (DE)
University of California Los Angeles (UCLA)
United States (USA) (US)
How to cite
APA:
Beis, D., Bartman, T., Jin, S.-W., Scott, I.C., D'Amico, L., Ober, E.A.,... Jungblut, B. (2005). Genetic and cellular analyses of zebrafish atrioventricular cushion and valve development. Development, 132(18), 4193-4204. https://doi.org/10.1242/dev.01970
MLA:
Beis, Dimitris, et al. "Genetic and cellular analyses of zebrafish atrioventricular cushion and valve development." Development 132.18 (2005): 4193-4204.
BibTeX: Download