The Spinster Homolog, Two of Hearts, Is Required for Sphingosine 1-Phosphate Signaling in Zebrafish

Osborne N, Brand-Arzamendi K, Ober EA, Jin SW, Verkade H, Holtzman NG, Yelon D, Stainier DYR (2008)

Publication Type: Journal article

Publication year: 2008

Journal

Current Biology Elsevier (Cell Press)

Book Volume: 18

Pages Range: 1882-1888

Journal Issue: 23

DOI: 10.1016/j.cub.2008.10.061

Abstract

The bioactive lipid sphingosine 1-phosphate (S1P) and its G protein-coupled receptors play critical roles in cardiovascular, immunological, and neural development and function [1-6]. Despite its importance, many questions remain about S1P signaling, including how S1P, which is synthesized intracellularly, is released from cells. Mutations in the zebrafish gene encoding the S1P receptor Miles Apart (Mil)/S1P2 disrupt the formation of the primitive heart tube [5]. We find that mutations of another zebrafish locus, two of hearts (toh), cause phenotypes that are morphologically indistinguishable from those seen in mil/s1p2 mutants. Positional cloning of toh reveals that it encodes a member of the Spinster-like family of putative transmembrane transporters. The biological functions of these proteins are poorly understood, although phenotypes of the Drosophila spinster and zebrafish not really started mutants suggest that these proteins may play a role in lipid trafficking [7, 8]. Through gain- and loss-of-function analyses, we show that toh is required for signaling by S1P2. Further evidence indicates that Toh is involved in the trafficking or cellular release of S1P. © 2008 Elsevier Ltd. All rights reserved.

Authors with CRIS profile

Elke Ober

Involved external institutions

University of California San Francisco (UCSF)

United States (USA) (US)

How to cite

APA:

Osborne, N., Brand-Arzamendi, K., Ober, E.A., Jin, S.-W., Verkade, H., Holtzman, N.G.,... Stainier, D.Y.R. (2008). The Spinster Homolog, Two of Hearts, Is Required for Sphingosine 1-Phosphate Signaling in Zebrafish. Current Biology, 18(23), 1882-1888. https://doi.org/10.1016/j.cub.2008.10.061

MLA:

Osborne, Nick, et al. "The Spinster Homolog, Two of Hearts, Is Required for Sphingosine 1-Phosphate Signaling in Zebrafish." Current Biology 18.23 (2008): 1882-1888.

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