Koltowska K, Apitz H, Stamataki D, Hirst EM, Verkade H, Salecker I, Ober EA (2013)
Publication Type: Journal article
Publication year: 2013
Book Volume: 140
Pages Range: 1912-1918
Journal Issue: 9
DOI: 10.1242/dev.093583
Tightly controlled DNA replication and RNA transcription are essential for differentiation and tissue growth in multicellular organisms. Histone chaperones, including the FACT (facilitates chromatin transcription) complex, are central for these processes and act by mediating DNA access through nucleosome reorganisation. However, their roles in vertebrate organogenesis are poorly understood. Here, we report the identification of zebrafish mutants for the gene encoding Structure specific recognition protein 1a (Ssrp1a), which, together with Spt16, forms the FACT heterodimer. Focussing on the liver and eye, we show that zygotic Ssrp1a is essential for proliferation and differentiation during organogenesis. Specifically, gene expression indicative of progressive organ differentiation is disrupted and RNA transcription is globally reduced. Ssrp1a-deficient embryos exhibit DNA synthesis defects and prolonged S phase, uncovering a role distinct from that of Spt16, which promotes G
APA:
Koltowska, K., Apitz, H., Stamataki, D., Hirst, E.M., Verkade, H., Salecker, I., & Ober, E.A. (2013). Ssrp1a controls organogenesis by promoting cell cycle progression and RNA synthesis. Development, 140(9), 1912-1918. https://doi.org/10.1242/dev.093583
MLA:
Koltowska, Katarzyna, et al. "Ssrp1a controls organogenesis by promoting cell cycle progression and RNA synthesis." Development 140.9 (2013): 1912-1918.
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