Wireless closed-loop optogenetics across the entire dorsoventral spinal cord in mice
Kathe C, Michoud F, Schönle P, Rowald A, Brun N, Ravier J, Furfaro I, Paggi V, Kim K, Soloukey S, Asboth L, Hutson TH, Jelescu I, Philippides A, Alwahab N, Gandar J, Huber D, De Zeeuw CI, Barraud Q, Huang Q, Lacour SP, Courtine G (2022)
Publication Type: Journal article
Publication year: 2022
Journal
Book Volume: 40
Pages Range: 198-208
Journal Issue: 2
DOI: 10.1038/s41587-021-01019-x
Abstract
Optoelectronic systems can exert precise control over targeted neurons and pathways throughout the brain in untethered animals, but similar technologies for the spinal cord are not well established. In the present study, we describe a system for ultrafast, wireless, closed-loop manipulation of targeted neurons and pathways across the entire dorsoventral spinal cord in untethered mice. We developed a soft stretchable carrier, integrating microscale light-emitting diodes (micro-LEDs), that conforms to the dura mater of the spinal cord. A coating of silicone–phosphor matrix over the micro-LEDs provides mechanical protection and light conversion for compatibility with a large library of opsins. A lightweight, head-mounted, wireless platform powers the micro-LEDs and performs low-latency, on-chip processing of sensed physiological signals to control photostimulation in a closed loop. We use the device to reveal the role of various neuronal subtypes, sensory pathways and supraspinal projections in the control of locomotion in healthy and spinal-cord injured mice.
Involved external institutions
Eidgenössische Technische Hochschule Zürich (ETHZ) / Swiss Federal Institute of Technology in Zurich
Switzerland (CH)
École Polytechnique Fédérale de Lausanne (EPFL)
Switzerland (CH)
Erasmus University Medical Center (MC)
Netherlands (NL)
University of Geneva / Université de Genève (UNIGE)
Switzerland (CH)
Switzerland (CH)
École Polytechnique Fédérale de Lausanne (EPFL)
Switzerland (CH)
Erasmus University Medical Center (MC)
Netherlands (NL)
University of Geneva / Université de Genève (UNIGE)
Switzerland (CH)
How to cite
APA:
Kathe, C., Michoud, F., Schönle, P., Rowald, A., Brun, N., Ravier, J.,... Courtine, G. (2022). Wireless closed-loop optogenetics across the entire dorsoventral spinal cord in mice. Nature Biotechnology, 40(2), 198-208. https://doi.org/10.1038/s41587-021-01019-x
MLA:
Kathe, Claudia, et al. "Wireless closed-loop optogenetics across the entire dorsoventral spinal cord in mice." Nature Biotechnology 40.2 (2022): 198-208.
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