Self-Quenched Fluorophore-DNA Labels for Super-Resolution Fluorescence Microscopy
Kessler LF, Balakrishnan A, Menche T, Wang D, Li Y, Mantel M, Glogger M, Dietz MS, Heilemann M (2024)
Publication Type: Journal article
Publication year: 2024
Journal
Abstract
Protein labeling through transient and repetitive hybridization of short, fluorophore-labeled DNA oligonucleotides has become widely applied in various optical super-resolution microscopy methods. The main advantages are multitarget imaging and molecular quantification. A challenge is the high background signal originating from the presence of unbound fluorophore-DNA labels in solution. Here, we report the self-quenching of fluorophore dimers conjugated to DNA oligonucleotides as a general concept to reduce the fluorescence background. Upon hybridization, the fluorescence signals of both fluorophores are restored. We expand the toolbox of fluorophores suitable for self-quenching and report their spectra and hybridization equilibria. We apply self-quenched fluorophore-DNA labels to stimulated emission depletion microscopy and single-molecule localization microscopy and report improved imaging performances.
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Germany (DE)How to cite
APA:
Kessler, L.F., Balakrishnan, A., Menche, T., Wang, D., Li, Y., Mantel, M.,... Heilemann, M. (2024). Self-Quenched Fluorophore-DNA Labels for Super-Resolution Fluorescence Microscopy. Journal of Physical Chemistry B. https://doi.org/10.1021/acs.jpcb.4c02065
MLA:
Kessler, Laurell F., et al. "Self-Quenched Fluorophore-DNA Labels for Super-Resolution Fluorescence Microscopy." Journal of Physical Chemistry B (2024).
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