Protocol for mapping the heterogeneous dendritic cell network across the murine tissue landscape via high-dimensional flow cytometry

Amon L, Seichter A, Vurnek D, Tchitashvili G, Heß I, Heger L, Lehmann C, Dudziak D (2024)

Publication Language: English

Publication Type: Journal article

Publication year: 2024

Journal

STAR Protocols Cell Press

Book Volume: 5

Article Number: 103151

Journal Issue: 3

DOI: 10.1016/j.xpro.2024.103151

Abstract

Dendritic cells (DCs) populate nearly all tissues and represent the central orchestrators of immunity. Here, we present a protocol for the mild but efficient preparation of single-cell suspensions from multiple murine tissues and the downstream analysis of the DC network via high-parameter flow cytometry. Additionally, we provide evaluation strategies that facilitate the stringent separation of the DC family from other myeloid cells, particularly macrophages and monocytes, and include an in-depth assessment of DC-intrinsic heterogeneity. For complete details on the use and execution of this protocol, please refer to Amon et al.1

Authors with CRIS profile

Lukas Amon Department of Dermatology Giorgi Tchitashvili Professur für die Biologie Dendritischer Zellen Lukas Heger Department of Dermatology Christian Lehmann Department of Dermatology Diana Dudziak Professur für die Biologie Dendritischer Zellen

Involved external institutions

Universitätsklinikum Jena DE Germany (DE)

How to cite

APA:

Amon, L., Seichter, A., Vurnek, D., Tchitashvili, G., Heß, I., Heger, L.,... Dudziak, D. (2024). Protocol for mapping the heterogeneous dendritic cell network across the murine tissue landscape via high-dimensional flow cytometry. STAR Protocols, 5(3). https://doi.org/10.1016/j.xpro.2024.103151

MLA:

Amon, Lukas, et al. "Protocol for mapping the heterogeneous dendritic cell network across the murine tissue landscape via high-dimensional flow cytometry." STAR Protocols 5.3 (2024).

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