Exosomal Serum Biomarkers as Predictors for Laryngeal Carcinoma †

Schuster J, Wendler O, Pesold VV, Koch M, Sievert M, Balk M, Rupp R, Müller SK (2024)

Publication Type: Journal article

Publication year: 2024

Journal

Cancers MDPI

Book Volume: 16

Article Number: 2028

Journal Issue: 11

DOI: 10.3390/cancers16112028

Abstract

Background: The lack of screening methods for LSCC is a critical issue, as treatment options and the treatment outcome greatly depend on the stage of LSCC at initial diagnosis. Therefore, the objective of this study was to identify potential exosomal serum biomarkers that can diagnose LSCC and distinguish between early- and late-stage disease. Methods: A multiplexed proteomic array was used to identify differentially expressed proteins in exosomes isolated from the serum samples of LSCC patients compared to the control group (septorhinoplasty, SRP). The most promising proteins for diagnosis and differentiation were calculated using biostatistical methods and were validated by immunohistochemistry (IHC), Western blots (WB), and ELISA. Results: Exosomal insulin-like growth factor binding protein 7 (IGFBP7) and Annexin A1 (ANXA1) were the most promising exosomal biomarkers for distinguishing between control and LSCC patients and also between different stages of LSCC (fold change up to 15.9, p < 0.001 for all). Conclusion: The identified proteins represent potentially novel non-invasive biomarkers. However, these results need to be validated in larger cohorts with a long-term follow-up. Exosomal biomarkers show a superior signal-to-noise ratio compared to whole serum and may therefore be an important tool for non-invasive biomarker profiling for laryngeal carcinoma in the future.

Authors with CRIS profile

Julius Schuster Department of Medicine 2 – Cardiology and Angiology Olaf Wendler Lehrstuhl für Hals-, Nasen- und Ohrenheilkunde Vanessa-Vivien Pesold Lehrstuhl für Hals-, Nasen- und Ohrenheilkunde Michael Koch Lehrstuhl für Hals-, Nasen- und Ohrenheilkunde Matti Sievert Department of Otorhinolaryngology – Head and Neck Surgery Matthias Balk Department of Otorhinolaryngology – Head and Neck Surgery Robin Rupp Department of Otorhinolaryngology – Head and Neck Surgery Sarina Müller-Hübner Department of Otorhinolaryngology – Head and Neck Surgery

How to cite

APA:

Schuster, J., Wendler, O., Pesold, V.-V., Koch, M., Sievert, M., Balk, M.,... Müller, S.K. (2024). Exosomal Serum Biomarkers as Predictors for Laryngeal Carcinoma †. Cancers, 16(11). https://doi.org/10.3390/cancers16112028

MLA:

Schuster, Julius, et al. "Exosomal Serum Biomarkers as Predictors for Laryngeal Carcinoma †." Cancers 16.11 (2024).

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