IgG sialylation occurs in B cells pre antibody secretion

Werner A, Hanić M, Zaitseva OO, Lauc G, Lux A, Nitschke L, Nimmerjahn F (2024)

Publication Type: Journal article

Publication year: 2024

Journal

Book Volume: 15

Article Number: 1402000

DOI: 10.3389/fimmu.2024.1402000

Abstract

Sialic acids as terminal sugar residues on cell surface or secreted proteins have many functional roles. In particular, the presence or absence of α2,6-linked sialic acid residues at the immunoglobulin G (IgG) Fc fragment can switch IgG effector functions from pro- to anti-inflammatory activity. IgG glycosylation is considered to take place inside the plasma blast/plasma cell while the molecule travels through the endoplasmic reticulum and Golgi apparatus before being secreted. However, more recent studies have suggested that IgG sialylation may occur predominantly post-antibody secretion. To what extent this extracellular IgG sialylation process contributes to overall IgG sialylation remains unclear, however. By generating bone marrow chimeric mice with a B cell-specific deletion of ST6Gal1, the key enzyme required for IgG sialylation, we now show that sialylation of the IgG Fc fragment exclusively occurs within B cells pre-IgG secretion. We further demonstrate that B cells expressing ST6Gal1 have a developmental advantage over B cells lacking ST6Gal1 expression and thus dominate the plasma cell pool and the resulting serum IgG population in mouse models in which both ST6Gal1-sufficient and -deficient B cells are present.

Authors with CRIS profile

Involved external institutions

Genos Glycoscience Research Laboratory Croatia (HR)

How to cite

APA:

Werner, A., Hanić, M., Zaitseva, O.O., Lauc, G., Lux, A., Nitschke, L., & Nimmerjahn, F. (2024). IgG sialylation occurs in B cells pre antibody secretion. Frontiers in Immunology, 15. https://doi.org/10.3389/fimmu.2024.1402000

MLA:

Werner, Anja, et al. "IgG sialylation occurs in B cells pre antibody secretion." Frontiers in Immunology 15 (2024).

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