Folate titanium (IV)-chitosan nanocomposites as drug delivery system for active-targeted cancer therapy: Design, HSA/GSH binding, mechanistic, and biological investigations
Alsaed A, Elshami FI, Ibrahim MM, Shereef H, Mohany H, van Eldik R, Shaban SY (2024)
Publication Type: Journal article, Review article
Publication year: 2024
Journal
Book Volume: 97
Article Number: 105826
DOI: 10.1016/j.jddst.2024.105826
Abstract
Folate Ti(IV)-chitosan nanocomposite (TiFA@CS NPs) were prepared from folate Ti(IV)-complex (TiFA) and chitosan (CS) with optimum particle size (175 ± 7 nm), high stability (ζ = −25.1 mV), and minimum hemolytic activity to target cells expressing folate receptors. The interaction with Human Serum Albumin (HSA), a target biomolecule, thought to be the primary pharmacological target of anticancer drugs, and Glutathione (GSH) considering that cisplatin resistance could be due to a reaction between cisplatin and GSH, were evaluated and revealed that, although TiFA@CS NPs show strong binding to HSA, compared to TiFA and FA, the former showed weaker binding to GSH and hence exhibits a lower resistant factor. Kinetic stability, affinity, and association constants of compounds-HSA were examined using a stopped-flow method, and the results show that the formulation of TiFA in TiFA@CS NPs alters the binding mechanism while also enhancing the coordination affinity and kinetic stability. Furthermore, in-vitro cytotoxicity revealed that active-targeting TiFA@CS NPs significantly inhibited FR-positive MCF-7 cells compared to FRs-negative A549 cells which in agreement with the binding constants and resistant factor. Moreover, in-vitro TiFA release assays using TiFA@CS NPs in 10 mM GSH, mimicking the tumor microenvironment, revealed increased TiFA release behavior. Together with its coordination affinity, release evaluations, and cytoxicity, TiFA@CS NPs showed good cell death and rapid tumor uptake, making them an excellent alternative for targeted drug delivery by FR.
Authors with CRIS profile
Involved external institutions
Kafrelsheikh University
Egypt (EG)
Taif University / جامعة الطائف
Saudi Arabia (SA)
Menoufia University (Minoufiya / Minufiya University) / جامعة المنوفية
Egypt (EG)
Egypt (EG)
Taif University / جامعة الطائف
Saudi Arabia (SA)
Menoufia University (Minoufiya / Minufiya University) / جامعة المنوفية
Egypt (EG)
How to cite
APA:
Alsaed, A., Elshami, F.I., Ibrahim, M.M., Shereef, H., Mohany, H., van Eldik, R., & Shaban, S.Y. (2024). Folate titanium (IV)-chitosan nanocomposites as drug delivery system for active-targeted cancer therapy: Design, HSA/GSH binding, mechanistic, and biological investigations. Journal of Drug Delivery Science and Technology, 97. https://doi.org/10.1016/j.jddst.2024.105826
MLA:
Alsaed, Amera, et al. "Folate titanium (IV)-chitosan nanocomposites as drug delivery system for active-targeted cancer therapy: Design, HSA/GSH binding, mechanistic, and biological investigations." Journal of Drug Delivery Science and Technology 97 (2024).
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