Characterization of a Human Respiratory Mucosa Model to Study Odorant Metabolism

Mérignac-Lacombe J, Kornbausch N, Sivarajan R, Boichot V, Berg K, Oberwinkler H, Saliba AE, Loos H, Ehret Kasemo T, Scherzad A, Bodem J, Büttner A, Neiers F, Erhard F, Hackenberg S, Heydel JM, Steinke M (2024)

Publication Type: Journal article

Publication year: 2024

Journal

Journal of agricultural and food chemistry

DOI: 10.1021/acs.jafc.4c00752

Abstract

Nasal xenobiotic metabolizing enzymes (XMEs) are important for the sense of smell because they influence odorant availability and quality. Since the major part of the human nasal cavity is lined by a respiratory mucosa, we hypothesized that this tissue contributed to nasal odorant metabolism through XME activity. Thus, we built human respiratory tissue models and characterized the XME profiles using single-cell RNA sequencing. We focused on the XMEs dicarbonyl and l-xylulose reductase, aldehyde dehydrogenase (ALDH) 1A1, and ALDH3A1, which play a role in food odorant metabolism. We demonstrated protein abundance and localization in the tissue models and showed the metabolic activity of the corresponding enzyme families by exposing the models to the odorants 3,4-hexandione and benzaldehyde. Using gas chromatography coupled with mass spectrometry, we observed, for example, a significantly higher formation of the corresponding metabolites 4-hydroxy-3-hexanone (39.03 ± 1.5%, p = 0.0022), benzyl alcohol (10.05 ± 0.88%, p = 0.0008), and benzoic acid (8.49 ± 0.57%, p = 0.0004) in odorant-treated tissue models compared to untreated controls (0 ± 0, 0.12 ± 0.12, and 0.18 ± 0.18%, respectively). This is the first study that reveals the XME profile of tissue-engineered human respiratory mucosa models and demonstrates their suitability to study nasal odorant metabolism.

Authors with CRIS profile

Nicole Kornbausch Lehrstuhl für Aroma- und Geruchsforschung Helene Loos Lehrstuhl für Aroma- und Geruchsforschung Andrea Büttner Lehrstuhl für Aroma- und Geruchsforschung

Additional Organisation(s)

FAU Forschungszentrum Neue Wirkstoffe (FAU NeW)

Involved external institutions

Centre des Sciences du Goût et de l’Alimentation CSGA FR France (FR) Universitätsklinikum Würzburg DE Germany (DE) Julius-Maximilians-Universität Würzburg DE Germany (DE) Helmholtz-Institut für RNA-basierte Infektionsforschung (HIRI) DE Germany (DE)

How to cite

APA:

Mérignac-Lacombe, J., Kornbausch, N., Sivarajan, R., Boichot, V., Berg, K., Oberwinkler, H.,... Steinke, M. (2024). Characterization of a Human Respiratory Mucosa Model to Study Odorant Metabolism. Journal of agricultural and food chemistry. https://doi.org/10.1021/acs.jafc.4c00752

MLA:

Mérignac-Lacombe, Jeanne, et al. "Characterization of a Human Respiratory Mucosa Model to Study Odorant Metabolism." Journal of agricultural and food chemistry (2024).

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