Nonlinear dynamics of femtosecond laser interaction with the central nervous system in zebrafish

Jun S, Jun S, Herbst A, Herbst A, Scheffter K, John N, Kolb J, Wehner D, Fattahi H (2024)

Publication Type: Journal article

Publication year: 2024

Journal

Communications Physics Springer Nature

Book Volume: 7

Article Number: 161

Journal Issue: 1

DOI: 10.1038/s42005-024-01653-2

Abstract

Understanding the photodamage mechanism underlying the highly nonlinear dynamic of femtosecond laser pulses at the second transparent window of tissue is crucial for label-free microscopy. Here, we report the identification of two cavitation regimes from 1030 nm pulses when interacting with the central nervous system in zebrafish. We show that at low repetition rates, the damage is confined due to plasma-based ablation and sudden local temperature rise. At high repetition rates, the damage becomes collateral due to plasma-mediated photochemistry. Furthermore, we investigate the role of fluorescence labels with linear and nonlinear absorption pathways in optical breakdown. To verify our findings, we examined cell death and cellular responses to tissue damage, including the recruitment of fibroblasts and immune cells after irradiation. These findings contribute to advancing the emerging nonlinear optical microscopy techniques and provide a strategy for inducing precise, and localized injuries using near-infrared femtosecond laser pulses.

Authors with CRIS profile

Soyeon Jun
Andreas Herbst
Kilian Scheffter Lehrstuhl für Experimentalphysik (Alfried Krupp von Bohlen und Halbach-Professur) Nora John Lehrstuhl für Tierphysiologie Julia Kolb Lehrstuhl für Tierphysiologie

Additional Organisation(s)

Sonderforschungsbereich 1540: Erforschung der Mechanik des Gehirns (EBM): Verständnis, Engineering und Nutzung mechanischer Eigenschaften und Signale in der Entwicklung, Physiologie und Pathologie des zentralen Nervensystems

Involved external institutions

Max-Planck-Institut für die Physik des Lichts (MPL) / Max Planck Institute for the Science of Light DE Germany (DE)

How to cite

APA:

Jun, S., Jun, S., Herbst, A., Herbst, A., Scheffter, K., John, N.,... Fattahi, H. (2024). Nonlinear dynamics of femtosecond laser interaction with the central nervous system in zebrafish. Communications Physics, 7(1). https://doi.org/10.1038/s42005-024-01653-2

MLA:

Jun, Soyeon, et al. "Nonlinear dynamics of femtosecond laser interaction with the central nervous system in zebrafish." Communications Physics 7.1 (2024).

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