Critical size defect regeneration using PEG-mediated BMP-2 gene delivery and the use of cell occlusive barrier membranes - the osteopromotive principle revisited

Wehrhan F, Amann KU, Molenberg A, Lutz R, Neukam FW, Schlegel KA (2013)

Publication Type: Journal article

Publication year: 2013

Journal

Clinical Oral Implants Research Wiley-Blackwell

Book Volume: 24

Pages Range: 910-920

Journal Issue: 8

DOI: 10.1111/j.1600-0501.2012.02489.x

Abstract

Objective: The objective of this study was to investigate if osseous regeneration can be accelerated by involvement of periosteal tissue. Bone defect regeneration could be accelerated by the involvement of periosteal tissue if osteogenic cell signalling is maintained within the defect. It was questioned if local cell-mediated BMP-2 gene delivery makes a cell occlusive membrane dispensable during bone critical size defect regeneration. Methods: PEG matrix (degradation time 10 days) and PEG membrane (degradation time 120 days) were used in the pig calvarial model. Cylindrical (1 × 1 cm) critical size defects (CSD) (9 per animal; 20 animals) were filled with: (i) particulated autologous bone, covered with PEG membrane (group 1); (ii) HA/TCP, covered with PEG membrane (group 2); (iii) HA/TCP, mixed with PEG matrix (group 3); and (iv) HA/TCP mixed with BMP-2-transfected osteoblasts and PEG matrix (group 4). BMP-2/4 gene transfer: liposomal in vitro transfection of BMP-2/V5-tag fusion-protein. Quantitative histomorphometry (toluidine blue staining) after 2, 4 and 12 weeks assessed bone formation. Semiquantitative immunohistochemistry estimated the expression of BMP-2, V5-tag, Runx-2 and Sox9. Results: PEG matrix embedded BMP-2 expressing cells presented higher bone formation (P < 0.05) than HA/TCP + PEG matrix defect filling or PEG membrane covering (HA/TCP filling) after 12 weeks. Highest expression of BMP-2, Runx-2 and lowest expression of fibrous tissue marker Sox9 was seen in the BMP-2 group. Conclusion: PEG matrix embedded BMP-2 expressing cells are capable to maintain osteogenic signalling and to accelerate osseous defect regeneration in absence of a cell occlusive membrane. © 2012 John Wiley & Sons A/S.

Authors with CRIS profile

Falk Wehrhan Department of Oral and Cranio-Maxillofacial Surgery Kerstin Ute Amann Professur für Nephropathologie Rainer Lutz Department of Oral and Cranio-Maxillofacial Surgery Friedrich Wilhelm Neukam Lehrstuhl für Zahnmedizin, insbesondere Mund-, Kiefer-, und Gesichtschirurgie

Involved external institutions

Straumann Holding AG CH Switzerland (CH)

How to cite

APA:

Wehrhan, F., Amann, K.U., Molenberg, A., Lutz, R., Neukam, F.W., & Schlegel, K.A. (2013). Critical size defect regeneration using PEG-mediated BMP-2 gene delivery and the use of cell occlusive barrier membranes - the osteopromotive principle revisited. Clinical Oral Implants Research, 24(8), 910-920. https://doi.org/10.1111/j.1600-0501.2012.02489.x

MLA:

Wehrhan, Falk, et al. "Critical size defect regeneration using PEG-mediated BMP-2 gene delivery and the use of cell occlusive barrier membranes - the osteopromotive principle revisited." Clinical Oral Implants Research 24.8 (2013): 910-920.

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