Induction and coexpression of latent transforming growth factor β-binding protein-1 and fibrillin-1 in experimental glomerulonephritis

Porst M, Daniel C, Plank C, Schocklmann HO, Reinhardt DP, Hartner A (2006)

Publication Language: English

Publication Type: Journal article

Publication year: 2006

Journal

Nephron Experimental Nephrology Karger

Book Volume: 102

Pages Range: e99-e104

Journal Issue: 3-4

DOI: 10.1159/000089688

Abstract

Background: Latent transforming growth factor-β-binding protein 1 (LTBP-1) and fibrillin-1 were shown to colocalize and interact in the extracellular matrix of the skin and vasculature. This interaction may regulate transforming growth factor-β (TGF-β) activity. TGF-β is an important progression factor for glomerular diseases. We hypothesized that LTBP-1 and fibrillin-1 are coexpressed in the glomerulus and upregulated during glomerulonephritis. Methods: Acute anti-Thy1.1 glomerulonephritis was induced with a single intravenous injection (1 mg/kg body weight) of a monoclonal anti-Thy1.1 antibody in rats. Real-time RT-PCR and immunohistochemical analyses for LTBP-1 and fibrillin-1 were performed. Results: Induction of glomerular LTBP-1 mRNA was detected on day 2 of disease, while mRNA for fibrillin-1 was already upregulated 1 day after induction of disease. Both LTBP-1 and fibrillin-1 showed a mesangial distribution. An expansion of the LTBP-1 and fibrillin-1-positive mesangial area was seen on day 6 of disease, when transient matrix accumulation was most prominent. On day 12 of disease, glomerular LTBP-1 and fibrillin-1 immunoreactivities had returned to control levels. In serial sections, some colocalization of LTBP-1 and fibrillin-1 was detected in control as well as in nephritic glomeruli. Conclusion: Mesangial expression of LTBP-1 and fibrillin-1 is induced early in experimental nephritis and LTBP-1 and fibrillin-1 are partially colocalized in the nephritic glomerulus. An interaction of these molecules could stabilize latent TGF-β complexes and thus attenuate the activation of TGF-β during this self-limited glomerular disease. Copyright © 2006 S. Karger AG.

Authors with CRIS profile

Christoph Daniel Department of Medicine 4 – Nephrology and Hypertension Christian Georg Plank Department of Paediatrics and Adolescent Medicine Andrea Hartner Department of Paediatrics and Adolescent Medicine

Involved external institutions

Christian-Albrechts-Universität zu Kiel DE Germany (DE) McGill University CA Canada (CA)

How to cite

APA:

Porst, M., Daniel, C., Plank, C., Schocklmann, H.O., Reinhardt, D.P., & Hartner, A. (2006). Induction and coexpression of latent transforming growth factor β-binding protein-1 and fibrillin-1 in experimental glomerulonephritis. Nephron Experimental Nephrology, 102(3-4), e99-e104. https://doi.org/10.1159/000089688

MLA:

Porst, Markus, et al. "Induction and coexpression of latent transforming growth factor β-binding protein-1 and fibrillin-1 in experimental glomerulonephritis." Nephron Experimental Nephrology 102.3-4 (2006): e99-e104.

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