Immunogenicity and diagnostic potential of synthetic antigenic cell surface glycans of leishmania

Anish C, Martin CE, Wahlbrink A, Bogdan C, Ntais P, Antoniou M, Seeberger PH (2013)

Publication Type: Journal article

Publication year: 2013

Journal

ACS Chemical Biology American Chemical Society

Book Volume: 8

Pages Range: 2412-2422

Journal Issue: 11

DOI: 10.1021/cb400602k

Abstract

Detection and quantification of pathogen-derived antigenic structures is a key method for the initial diagnosis and follow-up of various infectious diseases. Complex parasitic diseases such as leishmaniasis require highly sensitive and specific tests prior to treatment with potentially toxic drugs. To investigate the diagnostic potential of cell surface glycans found on Leishmania parasites, we identified diagnostically relevant glycan epitopes and used synthetic glycan microarrays to screen sera from infected humans and dogs. On the basis of the screening results, we selected a tetrasaccharide to generate anti-glycan antibodies. The corresponding tetrasaccharide-carrier protein conjugate was immunogenic in mice, and sera obtained from immunized mice specifically detected the Leishmania parasite. These results demonstrate how synthetic glycan arrays, in combination with immunological methods, help to identify promising carbohydrate antigens for pathogen detection. © 2013 American Chemical Society.

Authors with CRIS profile

Christian Bogdan Lehrstuhl für Mikrobiologie und Infektionsimmunologie

Involved external institutions

Max-Planck-Institut für Kolloid- und Grenzflächenforschung / Max Planck Institute of Colloids and Interfaces DE Germany (DE) University of Crete / Πανεπιστήμιο Κρήτης GR Greece (GR)

How to cite

APA:

Anish, C., Martin, C.E., Wahlbrink, A., Bogdan, C., Ntais, P., Antoniou, M., & Seeberger, P.H. (2013). Immunogenicity and diagnostic potential of synthetic antigenic cell surface glycans of leishmania. ACS Chemical Biology, 8(11), 2412-2422. https://doi.org/10.1021/cb400602k

MLA:

Anish, Chakkumkal, et al. "Immunogenicity and diagnostic potential of synthetic antigenic cell surface glycans of leishmania." ACS Chemical Biology 8.11 (2013): 2412-2422.

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