Holler C, Taylor RW, Schambony A, Möckl L, Sandoghdar V (2024)
Publication Type: Journal article
Publication year: 2024
DOI: 10.1038/s41592-024-02177-x
Delivery of very small amounts of reagents to the near-field of cells with micrometer spatial precision and millisecond time resolution is currently out of reach. Here we present μkiss as a micropipette-based scheme for brushing a layer of small molecules and nanoparticles onto the live cell membrane from a subfemtoliter confined volume of a perfusion flow. We characterize our system through both experiments and modeling, and find excellent agreement. We demonstrate several applications that benefit from a controlled brush delivery, such as a direct means to quantify local and long-range membrane mobility and organization as well as dynamical probing of intercellular force signaling.
APA:
Holler, C., Taylor, R.W., Schambony, A., Möckl, L., & Sandoghdar, V. (2024). A paintbrush for delivery of nanoparticles and molecules to live cells with precise spatiotemporal control. Nature methods. https://doi.org/10.1038/s41592-024-02177-x
MLA:
Holler, Cornelia, et al. "A paintbrush for delivery of nanoparticles and molecules to live cells with precise spatiotemporal control." Nature methods (2024).
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