Techniques to measure pilus retraction forces
Biais N, Higashi D, So M, Ladoux B (2012)
Publication Type: Book chapter / Article in edited volumes
Publication year: 2012
Journal
Publisher: Humana Press Inc.
Series: Methods in Molecular Biology
Book Volume: 799
Pages Range: 197-216
DOI: 10.1007/978-1-61779-346-2_13
Abstract
The importance of physical forces in biology is becoming more appreciated. Neisseria gonorrhoeaehas become a paradigm for the study of physical forces in the bacterial world. Cycles of elongations and retractions of Type IV pili enables N. gonorrhoeaebacteria to exert forces on its environment, forces that play major roles in the life cycle of this pathogen. In order to better understand the role of these forces, there is a need to fully characterize them. Here, we present two different techniques, optical tweezers and Polyacrylamide MicroPillars (PoMPs), for measuring pilus retraction forces. Initially designed for N. gonorrhoeae, these assays can be readily modified to study other pilus-bearing bacteria including Neisseria meningitidis.
Authors with CRIS profile
Involved external institutions
University of Paris 7 - Denis Diderot / Université Paris VII Denis Diderot
France (FR)
University of Arizona
United States (USA) (US)
Columbia University
United States (USA) (US)
France (FR)
University of Arizona
United States (USA) (US)
Columbia University
United States (USA) (US)
How to cite
APA:
Biais, N., Higashi, D., So, M., & Ladoux, B. (2012). Techniques to measure pilus retraction forces. In (pp. 197-216). Humana Press Inc..
MLA:
Biais, Nicolas, et al. "Techniques to measure pilus retraction forces." Humana Press Inc., 2012. 197-216.
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