E-cadherin-dependent stimulation of traction force at focal adhesions via the Src and PI3K signaling pathways
Jasaitis A, Estevez M, Heysch J, Ladoux B, Dufour S (2012)
Publication Type: Journal article
Publication year: 2012
Journal
Book Volume: 103
Pages Range: 175-184
Journal Issue: 2
DOI: 10.1016/j.bpj.2012.06.009
Abstract
The interplay between cadherin- and integrin-dependent signals controls cell behavior, but the precise mechanisms that regulate the strength of adhesion to the extracellular matrix remains poorly understood. We deposited cells expressing a defined repertoire of cadherins and integrins on fibronectin (FN)-coated polyacrylamide gels (FN-PAG) and on FN-coated pillars used as a micro-force sensor array (μFSA), and analyzed the functional relationship between these adhesion receptors to determine how it regulates cell traction force. We found that cadherin-mediated adhesion stimulated cell spreading on FN-PAG, and this was modulated by the substrate stiffness. We compared S180 cells with cells stably expressing different cadherins on μFSA and found that traction forces were stronger in cells expressing cadherins than in parental cells. E-cadherin-mediated contact and mechanical coupling between cells are required for this increase in cell-FN traction force, which was not observed in isolated cells, and required Src and PI3K activities. Traction forces were stronger in cells expressing type I cadherins than in cells expressing type II cadherins, which correlates with our previous observation of a higher intercellular adhesion strength developed by type I compared with type II cadherins. Our results reveal one of the mechanisms whereby molecular cross talk between cadherins and integrins upregulates traction forces at cell-FN adhesion sites, and thus provide additional insight into the molecular control of cell behavior. © 2012 by the Biophysical Society.
Authors with CRIS profile
Involved external institutions
National Center for Scientific Research / Centre national de la recherche scientifique (CNRS)
France (FR)
University of Paris 7 - Denis Diderot / Université Paris VII Denis Diderot
France (FR)
France (FR)
University of Paris 7 - Denis Diderot / Université Paris VII Denis Diderot
France (FR)
How to cite
APA:
Jasaitis, A., Estevez, M., Heysch, J., Ladoux, B., & Dufour, S. (2012). E-cadherin-dependent stimulation of traction force at focal adhesions via the Src and PI3K signaling pathways. Biophysical Journal, 103(2), 175-184. https://doi.org/10.1016/j.bpj.2012.06.009
MLA:
Jasaitis, Audrius, et al. "E-cadherin-dependent stimulation of traction force at focal adhesions via the Src and PI3K signaling pathways." Biophysical Journal 103.2 (2012): 175-184.
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