Multiarray cell stretching platform for high-magnification real-time imaging
Huang Y, Nguyen NT, Lok KS, Lee PPF, Su M, Wu M, Kocgozlu L, Ladoux B (2013)
Publication Type: Journal article
Publication year: 2013
Journal
Book Volume: 8
Pages Range: 543-553
Journal Issue: 4
DOI: 10.2217/nnm.13.45
Abstract
Aim: This article reports the development of a multiarray microchip with real-time imaging capability to apply mechanical strains onto monolayered cell cultures. Materials & methods: Cells were cultured on an 8-μ thick membrane that was positioned in the microscope focal plane throughout the stretching process. Each stretching unit was assembled from three elastomeric layers and a glass coverslip. A programmable pneumatic control system was developed to actuate this platform. Multiple stretching experiments were conducted with various cell lines. Results: The platform provides a maximum uniform strain of 69%. Acute and long-term cell morphological changes were observed. The supreme imaging capability was verified by real-time imaging of transfected COS-7 stretching and poststretching imaging of immunofluorescence- stained PTK2. Conclusion: The platform reported here is a powerful tool for studying mechanically induced physiological changes in cells. Such a device could be used in tissue regeneration for maintaining essential cell growth conditions. © 2013 Future Medicine Ltd.
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Singapore (SG)How to cite
APA:
Huang, Y., Nguyen, N.T., Lok, K.S., Lee, P.P.F., Su, M., Wu, M.,... Ladoux, B. (2013). Multiarray cell stretching platform for high-magnification real-time imaging. Nanomedicine, 8(4), 543-553. https://doi.org/10.2217/nnm.13.45
MLA:
Huang, Yuli, et al. "Multiarray cell stretching platform for high-magnification real-time imaging." Nanomedicine 8.4 (2013): 543-553.
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