Microfabricated Environments to Study Collective Cell Behaviors
Vedula SRK, Ravasio A, Anon E, Chen T, Peyret G, Ashraf M, Ladoux B (2014)
Publication Type: Book chapter / Article in edited volumes
Publication year: 2014
Journal
Publisher: Academic Press Inc.
Series: Methods in Cell Biology
Book Volume: 120
Pages Range: 235-252
DOI: 10.1016/B978-0-12-417136-7.00016-1
Abstract
Coordinated cell movements in epithelial layers are essential for proper tissue morphogenesis and homeostasis. Microfabrication techniques have proven to be very useful for studies of collective cell migration in vitro. In this chapter, we briefly review the use of microfabricated substrates in providing new insights into collective cell behaviors. We first describe the development of micropatterned substrates to study the influence of geometrical constraints on cell migration and coordinated movements. Then, we present an alternative method based on microfabricated pillar substrates to create well-defined gaps within cell sheets and study gap closure. We also provide a discussion that presents possible pitfalls and sheds light onto the important parameters that allow the study of long-term cell culture on substrates of well-defined geometries. © 2014 Elsevier Inc.
Authors with CRIS profile
Involved external institutions
National University of Singapore (NUS)
Singapore (SG)
University of Paris 7 - Denis Diderot / Université Paris VII Denis Diderot
France (FR)
Institut Jacques Monod (IJM)
France (FR)
Singapore (SG)
University of Paris 7 - Denis Diderot / Université Paris VII Denis Diderot
France (FR)
Institut Jacques Monod (IJM)
France (FR)
How to cite
APA:
Vedula, S.R.K., Ravasio, A., Anon, E., Chen, T., Peyret, G., Ashraf, M., & Ladoux, B. (2014). Microfabricated Environments to Study Collective Cell Behaviors. In (pp. 235-252). Academic Press Inc..
MLA:
Vedula, Sri Ram Krishna, et al. "Microfabricated Environments to Study Collective Cell Behaviors." Academic Press Inc., 2014. 235-252.
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