Strale PO, Duchesne L, Peyret G, Montel L, Nguyen T, Png E, Tampé R, Troyanovsky S, Hénon S, Ladoux B, Mège RM (2015)
Publication Type: Journal article
Publication year: 2015
Book Volume: 210
Pages Range: 333-346
Journal Issue: 2
Oligomerization of cadherins could provide the stability to ensure tissue cohesion. Cadherins mediate cell-cell adhesion by forming trans-interactions. They form cis-interactions whose role could be essential to stabilize intercellular junctions by shifting cadherin clusters from a fluid to an ordered phase. However, no evidence has been provided so far for cadherin oligomerization in cellulo and for its impact on cell-cell contact stability. Visualizing single cadherins within cell membrane at a nanometric resolution, we show that E-cadherins arrange in ordered clusters, providing the first demonstration of the existence of oligomeric cadherins at cell-cell contacts. Studying the consequences of the disruption of the cis-interface, we show that it is not essential for adherens junction formation. Its disruption, however, increased the mobility of junctional E-cadherin. This destabilization strongly affected E-cadherin anchoring to actin and cell-cell rearrangement during collective cell migration, indicating that the formation of oligomeric clusters controls the anchoring of cadherin to actin and cell-cell contact fluidity.
APA:
Strale, P.O., Duchesne, L., Peyret, G., Montel, L., Nguyen, T., Png, E.,... Mège, R.M. (2015). The formation of ordered nanoclusters controls cadherin anchoring to actin and cell-cell contact fluidity. The Journal of Cell Biology, 210(2), 333-346. https://doi.org/10.1083/jcb.201410111
MLA:
Strale, Pierre Olivier, et al. "The formation of ordered nanoclusters controls cadherin anchoring to actin and cell-cell contact fluidity." The Journal of Cell Biology 210.2 (2015): 333-346.
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