Adaptive rheology and ordering of cell cytoskeleton govern matrix rigidity sensing
Gupta M, Sarangi BR, Deschamps J, Nematbakhsh Y, Callan-Jones A, Margadant F, Mège RM, Lim CT, Voituriez R, Ladoux B (2015)
Publication Type: Journal article
Publication year: 2015
Journal
Book Volume: 6
Article Number: 7525
DOI: 10.1038/ncomms8525
Abstract
Matrix rigidity sensing regulates a large variety of cellular processes and has important implications for tissue development and disease. However, how cells probe matrix rigidity, and hence respond to it, remains unclear. Here, we show that rigidity sensing and adaptation emerge naturally from actin cytoskeleton remodelling. Our in vitro experiments and theoretical modelling demonstrate a biphasic rheology of the actin cytoskeleton, which transitions from fluid on soft substrates to solid on stiffer ones. Furthermore, we find that increasing substrate stiffness correlates with the emergence of an orientational order in actin stress fibres, which exhibit an isotropic to nematic transition that we characterize quantitatively in the framework of active matter theory. These findings imply mechanisms mediated by a large-scale reinforcement of actin structures under stress, which could be the mechanical drivers of substrate stiffness-dependent cell shape changes and cell polarity.
Authors with CRIS profile
Involved external institutions
National University of Singapore (NUS)
Singapore (SG)
Institut Jacques Monod (IJM)
France (FR)
National Center for Scientific Research / Centre national de la recherche scientifique (CNRS)
France (FR)
University of Paris 7 - Denis Diderot / Université Paris VII Denis Diderot
France (FR)
Singapore (SG)
Institut Jacques Monod (IJM)
France (FR)
National Center for Scientific Research / Centre national de la recherche scientifique (CNRS)
France (FR)
University of Paris 7 - Denis Diderot / Université Paris VII Denis Diderot
France (FR)
How to cite
APA:
Gupta, M., Sarangi, B.R., Deschamps, J., Nematbakhsh, Y., Callan-Jones, A., Margadant, F.,... Ladoux, B. (2015). Adaptive rheology and ordering of cell cytoskeleton govern matrix rigidity sensing. Nature Communications, 6. https://doi.org/10.1038/ncomms8525
MLA:
Gupta, Mukund, et al. "Adaptive rheology and ordering of cell cytoskeleton govern matrix rigidity sensing." Nature Communications 6 (2015).
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