Coordination between intra- and extracellular forces regulates focal adhesion dynamics

Sarangi BR, Gupta M, Doss BL, Tissot N, Lam F, Mège RM, Borghi N, Ladoux B (2017)


Publication Type: Journal article

Publication year: 2017

Journal

Book Volume: 17

Pages Range: 399-406

Journal Issue: 1

DOI: 10.1021/acs.nanolett.6b04364

Abstract

Focal adhesions (FAs) are important mediators of cell-substrate interactions. One of their key functions is the transmission of forces between the intracellular acto-myosin network and the substrate. However, the relationships between cell traction forces, FA architecture, and molecular forces within FAs are poorly understood. Here, by combining Förster resonance energy transfer (FRET)-based molecular force biosensors with micropillar-based traction force sensors and high-resolution fluorescence microscopy, we simultaneously map molecular tension across vinculin, a key protein in FAs, and traction forces at FAs. Our results reveal strong spatiotemporal correlations between vinculin tension and cell traction forces at FAs throughout a wide range of substrate stiffnesses. Furthermore, we find that molecular tension within individual FAs follows a biphasic distribution from the proximal (toward the cell nucleus) to distal end (toward the cell edge). Using super-resolution imaging, we show that such a distribution relates to that of FA proteins. On the basis of our experimental data, we propose a model in which FA dynamics results from tension changes along the FAs.

Authors with CRIS profile

Involved external institutions

How to cite

APA:

Sarangi, B.R., Gupta, M., Doss, B.L., Tissot, N., Lam, F., Mège, R.M.,... Ladoux, B. (2017). Coordination between intra- and extracellular forces regulates focal adhesion dynamics. Nano Letters, 17(1), 399-406. https://doi.org/10.1021/acs.nanolett.6b04364

MLA:

Sarangi, Bibhu Ranjan, et al. "Coordination between intra- and extracellular forces regulates focal adhesion dynamics." Nano Letters 17.1 (2017): 399-406.

BibTeX: Download