Contractile forces at tricellular contacts modulate epithelial organization and monolayer integrity
Salomon J, Gaston C, Magescas J, Duvauchelle B, Canioni D, Sengmanivong L, Mayeux A, Michaux G, Campeotto F, Lemale J, Viala J, Poirier F, Minc N, Schmitz J, Brousse N, Ladoux B, Goulet O, Delacour D (2017)
Publication Type: Journal article
Publication year: 2017
Journal
Book Volume: 8
Article Number: 13998
DOI: 10.1038/ncomms13998
Abstract
Monolayered epithelia are composed of tight cell assemblies that ensure polarized exchanges. EpCAM, an unconventional epithelial-specific cell adhesion molecule, is assumed to modulate epithelial morphogenesis in animal models, but little is known regarding its cellular functions. Inspired by the characterization of cellular defects in a rare EpCAM-related human intestinal disease, we find that the absence of EpCAM in enterocytes results in an aberrant apical domain. In the course of this pathological state, apical translocation towards tricellular contacts (TCs) occurs with striking tight junction belt displacement. These unusual cell organization and intestinal tissue defects are driven by the loss of actomyosin network homoeostasis and contractile activity clustering at TCs, yet is reversed by myosin-II inhibitor treatment. This study reveals that adequate distribution of cortical tension is crucial for individual cell organization, but also for epithelial monolayer maintenance. Our data suggest that EpCAM modulation protects against epithelial dysplasia and stabilizes human tissue architecture.
Authors with CRIS profile
Involved external institutions
Institut Jacques Monod (IJM)
France (FR)
Hôpital Necker-Enfants malades
France (FR)
Institut Curie
France (FR)
Institut de Génétique & Développement de Rennes (IGDR)
France (FR)
Hôpital Armand-Trousseau (AP-HP)
France (FR)
Hôpital Universitaire Robert-Debré
France (FR)
France (FR)
Hôpital Necker-Enfants malades
France (FR)
Institut Curie
France (FR)
Institut de Génétique & Développement de Rennes (IGDR)
France (FR)
Hôpital Armand-Trousseau (AP-HP)
France (FR)
Hôpital Universitaire Robert-Debré
France (FR)
How to cite
APA:
Salomon, J., Gaston, C., Magescas, J., Duvauchelle, B., Canioni, D., Sengmanivong, L.,... Delacour, D. (2017). Contractile forces at tricellular contacts modulate epithelial organization and monolayer integrity. Nature Communications, 8. https://doi.org/10.1038/ncomms13998
MLA:
Salomon, Julie, et al. "Contractile forces at tricellular contacts modulate epithelial organization and monolayer integrity." Nature Communications 8 (2017).
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