Myosin II isoforms play distinct roles in adherens junction biogenesis
Heuzé ML, Sankara Narayana GHN, D’Alessandro J, Cellerin V, Dang T, Williams DS, Van Hest JC, Marcq P, Mège RM, Ladoux B (2019)
Publication Type: Journal article
Publication year: 2019
Journal
Book Volume: 8
Article Number: e46599
DOI: 10.7554/eLife.46599
Abstract
Adherens junction (AJ) assembly under force is essential for many biological processes like epithelial monolayer bending, collective cell migration, cell extrusion and wound healing. The acto-myosin cytoskeleton acts as a major force-generator during the de novo formation and remodeling of AJ. Here, we investigated the role of non-muscle myosin II isoforms (NMIIA and NMIIB) in epithelial junction assembly. NMIIA and NMIIB differentially regulate biogenesis of AJ through association with distinct actin networks. Analysis of junction dynamics, actin organization, and mechanical forces of control and knockdown cells for myosins revealed that NMIIA provides the mechanical tugging force necessary for cell-cell junction reinforcement and maintenance. NMIIB is involved in E-cadherin clustering, maintenance of a branched actin layer connecting E-cadherin complexes and perijunctional actin fibres leading to the building-up of anisotropic stress. These data reveal unanticipated complementary functions of NMIIA and NMIIB in the biogenesis and integrity of AJ.
Authors with CRIS profile
Involved external institutions
Université Sorbonne Paris Cité
France (FR)
Swansea University
United Kingdom (GB)
Eindhoven University of Technology / Technische Universiteit Eindhoven (TU/e)
Netherlands (NL)
Laboratory of Physics and Mechanics of Heterogeneous Media / Physique et Mécanique des Milieux Hétérogènes (PMMH)
France (FR)
France (FR)
Swansea University
United Kingdom (GB)
Eindhoven University of Technology / Technische Universiteit Eindhoven (TU/e)
Netherlands (NL)
Laboratory of Physics and Mechanics of Heterogeneous Media / Physique et Mécanique des Milieux Hétérogènes (PMMH)
France (FR)
How to cite
APA:
Heuzé, M.L., Sankara Narayana, G.H.N., D’Alessandro, J., Cellerin, V., Dang, T., Williams, D.S.,... Ladoux, B. (2019). Myosin II isoforms play distinct roles in adherens junction biogenesis. eLife, 8. https://doi.org/10.7554/eLife.46599
MLA:
Heuzé, Mélina L., et al. "Myosin II isoforms play distinct roles in adherens junction biogenesis." eLife 8 (2019).
BibTeX: Download