Neutrophil extracellular traps and DNases orchestrate formation of peritoneal adhesions.

Elrod J, Heuer A, Knopf J, Schoen J, Schönfeld L, Trochimiuk M, Stiel C, Appl B, Raluy LP, Saygi C, Zlatar L, Hosari S, Royzman D, Winkler T, Lochnit G, Leppkes M, Grützmann R, Schett G, Tomuschat C, Reinshagen K, Herrmann M, Fuchs TA, Boettcher M (2023)

Publication Type: Journal article

Publication year: 2023

Journal

iScience Elsevier

Book Volume: 26

Journal Issue: 12

DOI: 10.1016/j.isci.2023.108289

Abstract

Peritoneal adhesions are poorly understood but highly prevalent conditions that can cause intestinal obstruction and pelvic pain requiring surgery. While there is consensus that stress-induced inflammation triggers peritoneal adhesions, the molecular processes of their formation still remain elusive. We performed murine models and analyzed human samples to monitor the formation of adhesions and the treatment with DNases. Various molecular analyses were used to evaluate the adhesions. The experimental peritoneal adhesions of the murine models and biopsy material from humans are largely based on neutrophil extracellular traps (NETs). Treatment with DNASE1 (Dornase alfa) and the human DNASE1L3 analog (NTR-10), significantly reduced peritoneal adhesions in experimental models. We conclude that NETs serve as essential scaffold for the formation of adhesions; DNases interfere with this process. Herein, we show that therapeutic application of DNases can be employed to prevent the formation of murine peritoneal adhesions. If this can be translated into the human situation requires clinical studies.

Authors with CRIS profile

Janina Schoen Department of Medicine 3 – Rheumatology and Immunology Leticija Zlatar Department of Medicine 3 – Rheumatology and Immunology Sami Hosari Department of Surgery Dmytro Royzman Department of Immune Modulation Thomas Winkler Professur für Genetik Moritz Leppkes Department of Medicine 1 – Gastroenterology, Pneumology and Endocrinology Robert Grützmann Lehrstuhl für Allgemein- und Viszeralchirurgie Georg Schett Lehrstuhl für Innere Medizin III Martin Herrmann Department of Medicine 3 – Rheumatology and Immunology

Involved external institutions

Universitätsklinikum Hamburg-Eppendorf (UKE) DE Germany (DE) Universitätsklinikum Mannheim DE Germany (DE) Justus-Liebig-Universität Gießen DE Germany (DE)

How to cite

APA:

Elrod, J., Heuer, A., Knopf, J., Schoen, J., Schönfeld, L., Trochimiuk, M.,... Boettcher, M. (2023). Neutrophil extracellular traps and DNases orchestrate formation of peritoneal adhesions. iScience, 26(12). https://doi.org/10.1016/j.isci.2023.108289

MLA:

Elrod, Julia, et al. "Neutrophil extracellular traps and DNases orchestrate formation of peritoneal adhesions." iScience 26.12 (2023).

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