Tff3 Deficiency Differentially Affects the Morphology of Male and Female Intestines in a Long-Term High-Fat-Diet-Fed Mouse Model.
Šešelja K, Bazina I, Vrecl M, Farger J, Schicht M, Paulsen F, Baus Lončar M, Pirman T (2023)
Publication Type: Journal article
Publication year: 2023
Journal
Book Volume: 24
Journal Issue: 22
Abstract
Trefoil factor family protein 3 (Tff3) protects the gastrointestinal mucosa and has a complex mode of action in different tissues. Here, we aimed to determine the effect of Tff3 deficiency on intestinal tissues in a long-term high-fat-diet (HFD)-fed model. A novel congenic strain without additional metabolically relevant mutations (Tff3-/-/C57Bl6NCrl strain, male and female) was used. Wild type (Wt) and Tff3-deficient mice of both sexes were fed a HFD for 36 weeks. Long-term feeding of a HFD induces different effects on the intestinal structure of Tff3-deficient male and female mice. For the first time, we found sex-specific differences in duodenal morphology. HFD feeding reduced microvilli height in Tff3-deficient females compared to that in Wt females, suggesting a possible effect on microvillar actin filament dynamics. These changes could not be attributed to genes involved in ER and oxidative stress, apoptosis, or inflammation. Tff3-deficient males exhibited a reduced cecal crypt depth compared to that of Wt males, but this was not the case in females. Microbiome-related short-chain fatty acid content was not affected by Tff3 deficiency in HFD-fed male or female mice. Sex-related differences due to Tff3 deficiency imply the need to consider both sexes in future studies on the role of Tff in intestinal function.
Involved external institutions
Rudjer Boskovic Institute (RBI) / Institut Ruđer Bošković (IRB)
Croatia (HR)
University of Ljubljana (UL) / Univerza v Ljubljani
Slovenia (SI)
Croatia (HR)
University of Ljubljana (UL) / Univerza v Ljubljani
Slovenia (SI)
How to cite
APA:
Šešelja, K., Bazina, I., Vrecl, M., Farger, J., Schicht, M., Paulsen, F.,... Pirman, T. (2023). Tff3 Deficiency Differentially Affects the Morphology of Male and Female Intestines in a Long-Term High-Fat-Diet-Fed Mouse Model. International Journal of Molecular Sciences, 24(22). https://dx.doi.org/10.3390/ijms242216342
MLA:
Šešelja, Kate, et al. "Tff3 Deficiency Differentially Affects the Morphology of Male and Female Intestines in a Long-Term High-Fat-Diet-Fed Mouse Model." International Journal of Molecular Sciences 24.22 (2023).
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