Evaluation of a fluorescence endoscope in murine in-vivo auto-fluorescence glioma models
Senger S, Linsler S, Sippl C, Oertel J (2021)
Publication Type: Journal article
Publication year: 2021
Journal
Book Volume: 237
Article Number: 151746
DOI: 10.1016/j.aanat.2021.151746
Abstract
Introduction: The value of extended and radical resection of high grade gliomas remains controversial, but the neurosurgical procedure is still vital for effective cancer treatment. Fluorescence guided surgery provides aggressive resection within the tumor margins even on microscopic levels. Aim of this study was to evaluate if a new developed fluorescence endoscope can improve intraoperative vision and tumor delineation. Methods: An autofluoresence C6 glioma cell line was established via GFP-transfection. These GFP-C6 glioma cells were transplanted both in a dorsal skinfold chamber of the mouse and orthotopically in a cranial window chamber of the mouse. After five days, tumors were examinated by intravital fluorescence microscopy, a standard fluorescence operation microscope and a fluorescence endoscope. Images were compared in terms of visualization, magnification and delineation of tumor cells from host tissue. Results: The fluorescence endoscope showed improved image quality and higher magnifications compared to the operation microscope. Even smallest tumor extensions were visualized by the fluorescence endoscope nearly reaching the quality of an intravital fluorescence microscope. Conclusions: In summary better visualization can improve the intraoperative decision making of the surgeons. So endoscopic assistance can be seen as a promising tool for the fluorescence guided resection of high grade gliomas in the next years.
Authors with CRIS profile
Involved external institutions
Germany (DE)How to cite
APA:
Senger, S., Linsler, S., Sippl, C., & Oertel, J. (2021). Evaluation of a fluorescence endoscope in murine in-vivo auto-fluorescence glioma models. Annals of Anatomy-Anatomischer Anzeiger, 237. https://doi.org/10.1016/j.aanat.2021.151746
MLA:
Senger, Sebastian, et al. "Evaluation of a fluorescence endoscope in murine in-vivo auto-fluorescence glioma models." Annals of Anatomy-Anatomischer Anzeiger 237 (2021).
BibTeX: Download