Burggraf R, Albert M (2024)
Publication Type: Journal article
Publication year: 2024
Book Volume: 2731
Pages Range: 217-230
DOI: 10.1007/978-1-0716-3511-7_16
In-vivo cross-linking of biotinylated peptides is a technique to analyze the interaction of small proteins or peptide ligands with their corresponding receptors. Here, we describe an in-vivo method in which leaves of living plants, transiently expressing receptor proteins, are infiltrated with biotinylated peptides. The interaction between ligand and receptor is irreversibly fixed by the infiltration of a cross-linking agent. Subsequently, co-immunoprecipitation is used to pull down the receptor-ligand pair. After western blotting, the biotin tag of the ligand peptide cross-linked to the receptor can be detected by streptavidin-AP conjugate on the membrane.
APA:
Burggraf, R., & Albert, M. (2024). In-vivo Cross-linking of Biotinylated Peptide Ligands to Cell Surface Receptors. Methods in Molecular Biology, 2731, 217-230. https://dx.doi.org/10.1007/978-1-0716-3511-7_16
MLA:
Burggraf, Ronja, and Markus Albert. "In-vivo Cross-linking of Biotinylated Peptide Ligands to Cell Surface Receptors." Methods in Molecular Biology 2731 (2024): 217-230.
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