Zhang J, Straehle J, Joseph K, Neidert N, Behringer S, Göldner J, Vlachos A, Prinz M, Fung C, Beck J, Schnell O, Heiland DH, Ravi VM (2023)
Publication Type: Journal article
Publication year: 2023
Book Volume: 4
Article Number: 102383
Journal Issue: 3
DOI: 10.1016/j.xpro.2023.102383
Single-cell RNA-sequencing (scRNA-seq) is becoming a ubiquitous method in profiling the cellular transcriptomes of both malignant and non-malignant cells from the human brain. Here, we present a protocol to isolate viable tumor cells from human ex vivo glioblastoma cultures for single-cell transcriptomic analysis. We describe steps including surgical tissue collection, sectioning, culturing, primary tumor cells inoculation, growth tracking, fluorescence-based cell sorting, and population-enriched scRNA-seq. This comprehensive methodology empowers in-depth understanding of brain tumor biology at the single-cell level. For complete details on the use and execution of this protocol, please refer to Ravi et al.1
APA:
Zhang, J., Straehle, J., Joseph, K., Neidert, N., Behringer, S., Göldner, J.,... Ravi, V.M. (2023). Isolation and profiling of viable tumor cells from human ex vivo glioblastoma cultures through single-cell transcriptomics. STAR Protocols, 4(3). https://doi.org/10.1016/j.xpro.2023.102383
MLA:
Zhang, Junyi, et al. "Isolation and profiling of viable tumor cells from human ex vivo glioblastoma cultures through single-cell transcriptomics." STAR Protocols 4.3 (2023).
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