Analyzing Fcγ-Receptor Interactions on Monocytes with the Proximity Ligation Assay (PLA)

Kara S, Nimmerjahn F (2024)

Publication Type: Journal article

Publication year: 2024

Journal

Methods in Molecular Biology Humana Press

Book Volume: 2713

Pages Range: 377-388

DOI: 10.1007/978-1-0716-3437-0_26

Abstract

Proximity ligation assays (PLA) enable the detection and characterization of protein interactions independent of protein abundance or genetic modifications. This technique exploits both antibody and DNA-binding features, providing high selectivity and sensitivity for protein recognition and visualization of single-protein molecules with high spatial accuracy. Here, we describe the general procedure for a direct PLA on splenic monocytes to analyze FcγRIIb homodimerization. However, this method can be applied to other cells and receptors of interest.

Authors with CRIS profile

Sibel Kara-Kocak Lehrstuhl für Genetik Falk Nimmerjahn Lehrstuhl für Genetik

How to cite

APA:

Kara, S., & Nimmerjahn, F. (2024). Analyzing Fcγ-Receptor Interactions on Monocytes with the Proximity Ligation Assay (PLA). Methods in Molecular Biology, 2713, 377-388. https://doi.org/10.1007/978-1-0716-3437-0_26

MLA:

Kara, Sibel, and Falk Nimmerjahn. "Analyzing Fcγ-Receptor Interactions on Monocytes with the Proximity Ligation Assay (PLA)." Methods in Molecular Biology 2713 (2024): 377-388.

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