Sopel N, Müller-Deile J (2023)
Publication Type: Journal article
Publication year: 2023
Book Volume: 2664
Pages Range: 145-157
DOI: 10.1007/978-1-0716-3179-9_11
The zebrafish model has been used in many different fields of research because of its high homology to the human genome, its easy genetic manipulation, its high fecundity, and its rapid development. For glomerular diseases, zebrafish larvae have proven to be a versatile tool to study the contribution of different genes, because the zebrafish pronephros is very comparable to the human kidney in function and ultrastructure. Here we describe the principle and use of a simple screening assay based on the measurement of the fluorescence in the retinal vessel plexus of the Tg(l-fabp:DBP:eGFP) zebrafish line ("eye assay") to indirectly determine proteinuria as a hallmark of podocyte dysfunction. Furthermore, we illustrate how to analyze the obtained data and outline methods to attribute the findings to podocyte impairment.
APA:
Sopel, N., & Müller-Deile, J. (2023). Zebrafish Model to Study Podocyte Function Within the Glomerular Filtration Barrier. Methods in Molecular Biology, 2664, 145-157. https://dx.doi.org/10.1007/978-1-0716-3179-9_11
MLA:
Sopel, Nina, and Janina Müller-Deile. "Zebrafish Model to Study Podocyte Function Within the Glomerular Filtration Barrier." Methods in Molecular Biology 2664 (2023): 145-157.
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